[ccp4bb] Transcription start site

Hi All I am trying to code gene prediction. I am looking for the consensus pattern for transcription start site, pribnov bow, ribosomal binding site and so on. I am looking some pattern which are variable and I could not make any conclusion. If anyone can provide all consensus pattern for eukar

[ccp4bb] X-server failed after installing coot

Dear All I am running Linux CentOS in my system. Recently I installed latest version of coot on my system. After successful installation, I restarted the system. But failed to recover Xserver screen. I was trying to restart Xserver from command mode but failed with following error message. "Yo

[ccp4bb] Super protein wire model bender

Dear Friends I am looking for the provider details of instrument - Byron Bender (or super protein wire model bender), which is used to build protein wire model using Torsion angle for teaching purpose. I could not find any such provider. I greatly appreciate your help Thank you Dr. B. Syed

[ccp4bb] Primer design

Hello All I am interested in cloning a gene from a plant. I searched the database only partial sequence is available, ie: for 160 residues only. The full length of the protein is around 570 residues. I designed forward primer and I have no clue to design reverse primer. Any help Thank you Sy

[ccp4bb] CM Sephadex C50

Hello All I am using CM Sephadex C50 column for my protein. I equilibrated column with MOPS buffer pH 6.0. But during elution I am using only NaCl2. This looks like changing the bed height. Before starting elution the bed height was around 60cm. After starting elution the bed height decreased a

Re: [ccp4bb] Skin on drops

In my practice, I never worried about the skin. You need extra care while you mount the crystal. In my case I have used the skin to mount my crystals without any problem. In fact it was much helpful. While mounting, you should not take the crystal covered with the skin. Best Syed

[ccp4bb] CNS download Info

Dear All Sorry to bother you by writing about CNS here. I am trying to contact Brunger to get the downlad information throguh my official id. Yet I am not getting any response. Could any one help? Thanks in advance Syed

Re: [ccp4bb] Off-topic: hollow protein crystals

Andre I had same kind of crystal like in your picture. It diffracted around 2.7A. Even I had some crystals where one end was closed. Both hollow crystal or the closed end (part) crystal diffracted same. With regards Syed --- On Thu, 6/3/10, Andre Ambrosio wrote: From: Andre Ambrosio Subj

Re: [ccp4bb] control of nucleation

Hi I have succeeded by using oil (such as parafin oil ... etc ) at reservoir on top of the reservoir solution. You have to try several trials to find optimum ratio. With regards Syed --- On Thu, 5/6/10, zq deng wrote: From: zq deng Subject: [ccp4bb] control of nucleation To: CCP4BB@JISCMA

Re: [ccp4bb] Cryo Vs crystal size

strem or propane etc2. did you try to flash anneal the larger crystal3. smeary diffraction from the big crystal or not ?4. how much residual solvent was around your crystal when freezing ? In general smaller crystals are anyhow better in my hands. Jürgen On Apr 14, 2010, at 5:36 PM, syed ibrahim

[ccp4bb] Cryo Vs crystal size

Hi All I had two crystals grown in same well, one is small and other is 10 times bigger. I treated both crystal in same cryo and same time. The smaller one diffracted to 2.5A and the bigger one to 6-7A. I was expecting the bigger one to diffract high resolution. I assume the bigger crystal mi