Hi
i have setup a crystallization of a complex formed by two different
proteins of molecular weights 53kD and 13kD. During purification of this
complex there was slight degradation band of 53KD protein as observed from
SDS PAGE but it did not effect complex formation. Crystals appeared after 8
mont
Hi
I am refining my 2 angstrom strucutre using phenix windows based software.
After many refinements my clashscore is not at all reducing and showing a
value of 12. Can anybody suggest how to reduce the clashscore. Is there any
technique to do it. Or i have to deal with each individual clashes
men
Hi friends
what is the maximum mosaicity value to work with. When i am doing Heavy
metal replacement my mosaicity value is around 1 - 1.2 after processing
through HKL. I don't know is it acceptable data. I have used mercuric
chloride for my protein and it has shown good results .However in case of
