[ccp4bb] Is it possible for the Tris buffer to strip the Zn ions from the Zinc Finger motif of a protein?

Date: Sat, 22 May 2010 11:17:41 +0800 From: rh_ibp2...@hotmail.com Subject: [ccp4bb] To: CCP4BB@JISCMAIL.AC.UK Dear all, Recently, I am working on a complex which includes two protein subunits. The interaction was based on the Zinc Finger motif of one protein. I co-purified the complex

[ccp4bb]

Dear all, Recently, I am working on a complex which includes two protein subunits. The interaction was based on the Zinc Finger motif of one protein. I co-purified the complex by nickel affinity column with one protein bearing a C terminal His tag and the other without any affinity tags. Ho

[ccp4bb] problem in expression of glycoproteins in baculovirus expression system

Dear all, Recently I am secretely expressing an ectodomain of a human membrane protein in insect cells. The expression level is moderately high but the target band in the SDS-PAGE gel seems smearing and this is cosistent with the western blot results. Does it result from the heterogeneous g

[ccp4bb] problems of co-crystallization of protein-DNA complex

Dear CCP4bbers, I am now working on a DNA binding protein and the purity of the protein is quite good, however the results of DLS showed that the protein aggregates terribly in quite a lot of different buffer conditions I tried and still no crystals can be obtained. So I am going to co-c