[ccp4bb] pMAL p2X

Dear AllThank you for your infornation regarding this vectorBest RegardsRana

[ccp4bb] pMAL -p2X vector

Dear CCP4Sorry for the question, does anyone work with the pMAL-p2X vector not c2X, because I am trying to find this vector for a long time now, and neither NEB of life technologies provide this vector. Does anyone know from where  I can get it or any company provides it, I would be grateful. Be

[ccp4bb] cryo-EM

Dear All Thank you for your suggestions and articles regarding the cryo-EM Best Regards Rana

[ccp4bb] cryo-EM

Dear CCP4 I wanted to ask has anyone tested 3D protein structure by cryo-electron microscopy? what is the suitable concentration required for this procedure Best Regards Rana

[ccp4bb] Thank you

Dear CCP4 Thank you for all your suggestions Best Regards Rana

Re: [ccp4bb] maltose binding protein

, is there anything that could maybe precipitate the MBP Best Regards Rana From: Mark J van Raaij To: rana ibd Sent: Thursday, March 27, 2014 11:35 AM Subject: Re: [ccp4bb] maltose binding protein did you try the maltose-resin? in principle it should bind

[ccp4bb] maltose binding protein

Dear CCP4 Does anyone know how to remove the maltose binding protein after cleavage from the target protein; I have tried gel filtration and ion exchange but with no luck, my protein is interacting with the MBP even after complete cleavage. I would be grateful for any help or suggestions Best Reg

[ccp4bb] cryoprotection condition

Dear CCP4 Thank you for your suggestions Best Regards Rana

[ccp4bb] cryoprotection condition

Dear CCP4 Does anyone know a good cryoprotection condition for these two conditions I would be grateful The first condition:  10% PEG 2, 20%PEG MME 550. 0.03M of NPS ( NPS is sodium nitrate, disodium hydrogen phosphate, and ammonium sulfate), 0.1MMES/Imadizole pH 6.5 The second condition: 20%

[ccp4bb] help

Dear ccp4    I wanted to ask if anyone has expressed DDB1 (Damaged DNA Binding protein)  or any other interacting partner using bacterial cells instead of insect or mammalian or yeast cells, I also wanted to ask if anyone has any experience with this binding partner interacting with a fusion

[ccp4bb] DDB1

Dear ccp4    I wanted to ask if anyone has expressed DDB1 (Damaged DNA Binding protein) using bacterial cells instead of insect or mammalian cells, I also wanted to ask if anyone has any experience with this binding partner interacting with a fusion protein (containing an MBP tag) , I would be grat

Re: [ccp4bb] Protein concentration for crystallization

Dear Debasish What do you mean by percentage? do you mean consentration? so if you mean cons. I think you should test you protein using a TCP kit to observe at what cons. would your protein  precipitate, this way you would verify the convinient cons.  for your protein before crystallization Best

[ccp4bb] Dear ccp4

Dear CCP4 Thank you for all your suggestions regarding my problem and will try all your ideas  that were offered best regards Rana

[ccp4bb] protein cleavage

Dear All    Thank you for all your replies, the buffer for the TEV protease that I have used contains 50mM Tris-HCl, 150mM NaCl, 1mM EDTA, and 1mM DTT at PH= 8.0. I have tried using this buffer without NaCl but the TEV protease precipitates when dialyzing over night, as for using glutathione (r

[ccp4bb] protein cleavage

Dear CCP4 I am having a problem with cleaving my fusion protein and I would be grateful if you advice me regarding this situation,  I have an MBP-DHBx fusion protein and I am trying to cleave it using TEV protease, I have tried different ratios and different temperatures  with different inc

[ccp4bb] Dear ccp4

Dear CCp4 Thank you for all your and information suggestions regarding question Best Regards Rana

[ccp4bb] dear ccp4

Dear ccp4    I am working on hbx (Hepatitis B x protein for both that duck and the human) I worked first on the dhbx which  contained the vector pRSET-A and I didn’t get any results, then I changed the vector ( thanks to many of you who provided me with information, papers and books) using p-MA

[ccp4bb] Dear ccp4

Dear ccp4    I am working on hbx (Hepatitis B x protein for both that duck and the human) I worked first on the dhbx which  contained the vector pRSET-A and I didn’t get any results, then I changed the vector ( thanks to many of you who provided me with information, papers and books) using p-MA

[ccp4bb] dear all

Dear all  yes my paalet is large enough and no my protein is not soluble ,and  I am using 37degrees before and after induction with IPTG,and the vector that I am using is pRSETA 3.2kd Best Regards Rana From: Kelly Daughtry To: rana ibd Cc: CCP4BB

[ccp4bb] dea all

Dear all I am expressing a 6xHis tagged in a dHBx protein in E.coli BL21 using LB madia, I am having problems with the expression which shows small amount of the protein , I also have problems with purification using NI-NTA by also having small amount even after extensive buffer exchange , Is i