Re: [ccp4bb] off-topic: protein losing FAD during purification

2014-03-14 Thread ramesh vandanapu
Dear All On similar lines any suggestions and tips on expression and purification of the proteins containing both FAD and NADPH would be really helpful. I recently tried to express and purify a protein containing these two ligands, but failed (aggregation of protein). my initial questions are

Re: [ccp4bb] PDB "crawler"

2013-03-20 Thread ramesh vandanapu
Hi Sebastino Both PDB and PBDe have facebook pages. PDBe updates the page weekly with some snippets as well. On Wed, Mar 20, 2013 at 3:41 PM, Boaz Shaanan wrote: > Hi, > > I think that what you're looking for (or close to it) is available on > the pdb site. Through your PDB login