My suggestions are
1 try complementary and non-complementary overhangs which can form
Watson-crick and/ or Hoogstein base pairing.
2. If it binds self-complementary duplexes then try them also.
3. Peg conditions with slight acidic pH are more suitable.
4. Divalent cation salts (Ca, Mg, Mn) in cryst
Hi Xiang.
If you are doing in vitro experiments like binding studies then try BODIPY
Fluorophores.
It conjugates the protein. Advantage is molecular weight of these BODIPY
Fluorophores is few hundreds Da.
Also, these dyes have well separable absorption and emission spectrums.
Good luck
Raj
On M
Hi Monica
If protein is Homo-tetramer then one can expect the identical binding
sites. I am also working on homo-dimeric protein which binds to DNA. I
used PRISM to estimate the binding affinity through flourescence
bindingmethod using “SATURATION and NON-LINEAR REGRESSION and ONE SITE
binding
Mo
Dear Fulvio Saccoccia
As you suspected it might be I4 instead of P4.
Since fractional coordinates are (0.5, 0.5, 0.479) and, 0.479 is almost 0.5.
Try to integrate and scale in I4 and see how chi-square behaves.
Raj
On Fri, Jun 7, 2013 at 11:17 AM, Fulvio Saccoccia <
fulvio.saccoc...@uniroma
