Hello people,
Thank you very much for so many replies you've gone to for me including
direct emails. This is a little summary of all the replies as a token of my
appreciation.
> It would take too long to list all the options here -
> however I would look at the earliest stages of your expressi
Dear all,
This is a question on how to cope with the protein that seems to form
massive aggregates in solution but enzymatically active.
I'm working on a protein whose molecular weight is around 70kDa and can be
divided into two domains (say A and B domains). We expressed this protein in
E.co