[ccp4bb] Summary - Active aggregates

2009-09-01 Thread ose toyoyuki
Hello people, Thank you very much for so many replies you've gone to for me including direct emails. This is a little summary of all the replies as a token of my appreciation. > It would take too long to list all the options here - > however I would look at the earliest stages of your expressi

[ccp4bb] Active aggregates?

2009-08-26 Thread ose toyoyuki
Dear all, This is a question on how to cope with the protein that seems to form massive aggregates in solution but enzymatically active. I'm working on a protein whose molecular weight is around 70kDa and can be divided into two domains (say A and B domains). We expressed this protein in E.co