[ccp4bb] Off Topic, How to refine carbohydate chain using CNS

Dear colleague, I am refining a structure containing a carbohydate chain using CNS, which is SIA(a2-3){Gal(b1-3)GalNac(b1-4)}Gal(b1-4)Glc. I bulit the model of the carbohydrate chain as five single carbohydrate like residues in peptides. The parameter and topology files are from

Re: [ccp4bb] Peptide on two-fold axis - was:[ccp4bb] PEG molecule crossing a two-fold crystallographic symmetry axis

rystallographic symmetry in coot. (Draw -> >Cell & Symmetry). > >Good luck! >Herman > > >-Original Message- >From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of lidefeng >Sent: Thursday, April 30, 2009 3:44 AM >To: CCP4BB@JISCMAIL.AC.UK &

Re: [ccp4bb] Peptide on two-fold axis - was:[ccp4bb] PEG molecule crossing a two-fold crystallographic symmetry axis

ach direction. > >Tim >-- >Tim Gruene >Institut fuer anorganische Chemie >Tammannstr. 4 >D-37077 Goettingen > >GPG Key ID = A46BEE1A > > >On Wed, 29 Apr 2009, lidefeng wrote: > >> Hi everyone, >> >> Following Chandrika's question,

Re: [ccp4bb] PEG molecule crossing a two-fold crystallographic symmetry axis

Hi everyone, Following Chandrika's question, what should I do if one peptide chain crosses a two-fold crystallographic symmetry axis? The peptide is not symmetric and the sidechain of one Se-Met (two after CS operation) is determined and conformed by MAD. Your sincerely 　　

[ccp4bb] Does it effort the refinement if no fully recored reflection?

no fully recored reflection. I wonder whether it is a serious problem for the final refinement, such as for R-free and R-work. Thank you! Your sincerely lidefeng [EMAIL PROTECTED] 　　2008-12-11 Defeng Li, Dr., Email