Re: [ccp4bb] EMBO Workshop on Molecular Neurobiology, 13th to 17th May 2024, Crete

- Jonathan Elegheert, PhD Team Leader CNRS Scientist Interdisciplinary Institute for NeuroScience (IINS) <https://www.iins.u-bordeaux.fr/> UMR5297 CNRS / UB Centre Broca Nouvelle-Aquitaine 146 rue Léo Saignat BP 61292 33076 Bordeaux Cedex <https

Re: [ccp4bb] Optimising a Nanobody interface computationally to improve binding

leads. Cheers Jonathan - Jonathan Elegheert, PhD Team Leader CNRS Scientist Interdisciplinary Institute for NeuroScience (IINS) <https://www.iins.u-bordeaux.fr/> UMR5297 CNRS / UB Centre Broca Nouvelle-Aquitaine 146 rue Léo S

[ccp4bb] Postdoctoral position at IINS, CNRS / University of Bordeaux, France

nathan - Jonathan Elegheert, PhD Team Leader Interdisciplinary Institute for NeuroScience (IINS) UMR5297 CNRS / UB University of Bordeaux Centre Broca Nouvelle-Aquitaine 146 rue Léo Saignat 33076 Bordeaux Cedex France Tel: +33-(0)5.33.51.48.57

Re: [ccp4bb] Non-specific disulfides in a secreted protein

Hi Tomas, In addition to all other suggestions, and as Zhijie suggests, a good alternative strategy to plasmid dilution may be inducible expression using the HEK293S GnTI– TetR cell line (PMID: 12370423) and a compatible expression vector such as pACMV-TetO (PMID: 22322218) or pHR-CMV-TetO2 (PM

Re: [ccp4bb] OFF TOPIC

wishes, Jonathan - Jonathan Elegheert, PhD Team Leader Interdisciplinary Institute for NeuroScience (IINS) UMR5297 CNRS / UB University of Bordeaux Centre Broca Nouvelle-Aquitaine 146 rue Léo Saignat 33076 Bordeaux Cedex France E

Re: [ccp4bb] PHENIX vs REFMAC refinement had me fooled

In addition to the remarks of Mark and Tim, could you make sure that you are refining in Refmac with the correct flag for the Rfree set (value = 0)? In Phenix, this is the opposite: the value is 1 for test reflections and 0 for work reflections. So going from a PHENIX environment to Refmac, you

Re: [ccp4bb] Merohedral twining for P212121. -

SHELXL dropped R/R-free and improved the GooF and least-squares refinement convergence, even though the twin fraction was rather low (~ 5%). HTH, Jonathan -- Jonathan Elegheert Ph.D. Student Unit for Structural Biology& Biophysics http://www.lprobe.ugent.be/xray.html Lab for Pro

[ccp4bb] Off-topic: unidentified density for N-terminal Thr modification

ow that sort of reactivity. Many thanks in advance if anyone has any pointers, Jonathan -- Jonathan Elegheert Ph.D. Student Ghent University

[ccp4bb] Detector pitch, roll, yaw

Dear bb, can somebody explain to me the exact definitions for detector roll, pitch and yaw? Can they be detector dependent or rather dependent on the beamline setup? Is there a relationship with the goniometer position? Many thanks in advance, Jonathan -- Jonathan Elegheert Ph.D. Student