Hello CCP4BBers:
Could some body send me a sample script to run ARCIMBALDO, the ab
initio protein structure determination software. Thanks a lot.
John
Hello All,
This is regarding twinning in a data set.
I collected a native data set to resolution, 1.8 A. I used XDS suite
to process and scale the data set. It scaled well in P622 and I found
systematic absence (l=6n present).
Hence thought the space group may be P6122/P6522. SFCHECK did not
Hello,
I wish to learn about self-rotation Patterson maps such as how to
interpret a map and find out non-crystallographic symmetry. Could you
suggest some good papers (for dummies), web-sites etc.
Thanks a lot for your help.
John
Hi All,
Apologies for this slight off topic, however this could very well be
the best bulletin board to seek help.
I need to calculate the electrostatic surface and make surface figures
for a protein at various pHs, say 4.0, 7.0 & 9.0. I'm doing this kind
of wok for the first time. Could you su
Hi All,
I wish to confirm that when we collect native dataset 0-360 for a
protein crystal (P1 space group) , the redundancy could very well be
>2 since many reciprocal points cross Ewald sphere twice, right ?
Thanks a lot.
John
ndard geometry condition. Could someone tell me what to do in this
situation. Should I make the occupancy of the oxygen zero. Any
comments would be gratefully appreciated.
Thanks very much.
John Peter
Hi CCP4ers,
Apologies for this off-topic question, but I believe someone in this
community could help me.
Suppose if I know the position of every 3rd or 4th or n-th unit (amino acid
or nucleotide or any moiety) of any helix (not just the protein & nucleic
acid helices), is it possible to generat
Dear Folks,
I recently grew crystals in the presence of 100 mM Imidazole pH 8.0 and 1
M Ammonium Phosphate. The protein was in 25 mM Hepes pH 7.4, 10 mM EDTA, 10
mM EGTA and 100 mM NaCl. I like to make heavy atom derivatives with Hg, Pt,
Sm, Au and Pb compounds. I think the imidazole will inter
