You would need to incorporate as much information you can find as possible to
make the 'best' prediction of domain boundaries. Typically, I would put
together all the following information in one multiple sequence alignment to
design domain constructs for structural studies.
- Known structural
In case someone do not know this feature or how to set it up, you can choose to
get the daily digest version of CCP4BB so that your mail box will not be filled
with mails that you can't find time to read individually. I have just changed
my settings at JISCMAIL web site. You would have to click
Hi Tania,
A classical way of superimposing two structures is to use lsq_e and lsq_i
commands in O . You can specify the regions that you want to match and
visualize the superimposed structures right away to see if they make sense or
not. O will also give you a pairwise sequence alignment and RM
I don't know much about Pichia expression system. You can consult
glycobiologists if you are 100% sure that no mistake was made during the
experiments.
Our lab has been using Drosophila S2 insect cell expression system. With this
system, you can maintain cell culture using roller bottles or fl
There are many cases in literature where the removal of N-linked glycosylations
sites reduced the expression levels of secreted proteins. I would recommend
removal of the N-linked glycans by enzymes (endo H, PNGase F, etc.) using the
wild-type proteins you have obtained and then run a gel or mas
Hi Hongmin,
I have used the anti-penta-His HRP conjugate antibody from Qiagen, and it works
very well. It comes as a kit which includes a special blocking buffer. You
don't need to use any secondary antibody, so it saves time. But you can only
use the diluted solution 1-2 times within 1-2 week
You can use APBS plugin in PyMol. See installation instruction at
http://www-personal.umich.edu/~mlerner/PyMOL/ or
http://www.pymolwiki.org/index.php/APBS
Holly
> Date: Tue, 8 Jan 2008 14:01:02 -0600
> From: [EMAIL PROTECTED]
> Subject: [ccp4bb] Any programs othe
