Hi,
I think I have new problems here with CNS. The structure contains a DNA with a
abasic dideoxyribose（1p,2p-dideoxyribofuranose-5p-phosphate，3DR）. But the
pdb genetated by generated_easy.inp lose the abasic site. I used the .param
and .top of 3DR on HIC-Up website. They work when the model is 3D
Dear Fred. and Martyn:
Thank you for your replies. I am new to CNS and I just followed the CNS
website protocols. I got your ideas. Since my resolution is 2A, I should skip
the bgroup refinement.
Hongjun Yu
Hi，
I am using CNS to refine the structure.But when I come to bgroup refinement,
the
produced bgroup.pdb have many residues with very high B factor. I read the
bgroup.inp, and found the item‘B-factor groups'with following description:
{* select atoms in group 1 - protein mainchain example *}
{==
Hi,
I am refining a protein complexed with a modified DNA(cordinates
obtained from published structure) with CNS. But I am stuck with the pdb
format problem. I followed the tutorial on CNS website and the first
step just went wrong when command 'fix_dna_rna' didn't work. I also
tried the normal DNA
