Dear Leo,
here's a little gedankenexperiment: imagine cross-linking those solutions
you have, say with glutaraldehyde. Would you say this will *improve*
solubility because there are now less molecules?
Best wishes,
Sebastiaan Werten.
> Dear all --
>
> I have a very "stupid" question/remark con
Fred,
it's not my intention to engage in any sort of discussion here about the
usefulness of science, in particular because I think we can all agree on
that one.
The point I was trying to make is that if young people in countries like
France turn away from science, this might not be because they
We all love science Fred, but are you sure you would want to encourage
young Frenchmen to start a career in it? After all, one's own glossy TV
channel is poor compensation for a lifetime of precariousness. I'm afraid
that if people in France choose to do an MBA instead, right now that may
not be ig
Dear Clemens,
sounds like typical pathology of plate-like crystals, which often suffer
from slight growth defects and possibly deformation during
handling/freezing.
If the Se-Met crystals are indeed easy to obtain in large numbers you may
want to test as many of them as you can, because there can
This story is rather puzzling indeed, and it is difficult to see why in
case of toxicity one would have transformation problems with that one
strain in particular.
Also, I am wondering how likely it is that a combination of toxicity and
leaky expression would lead to transformation problems in the
Hi Frank,
such interactions are certainly possible, in particular if additional
H-bonding side chains are available to stabilize the arrangement. I'm not
aware of any examples involving main chain carbonyls, but for a discussion
of carbonyl-carbonyl side chain interactions see the following paper:
Dear colleagues,
it appears that following the rejection of the software patent directive by
the European Parliament there is now another attempt going on to get
software patents consolidated, this time "through the back door".
Since the threat of software patents is relevant to the crystallograp
- Original Message -
From: Dominique BOURGEOIS
To: CCP4BB@JISCMAIL.AC.UK
Sent: Friday, January 04, 2008 4:18 PM
Subject: [ccp4bb] Post-doc position at the IBS, Grenoble, France
Kinetic protein crystallography of fluorescent proteins.
[...]
Candidates should have a strong expertise in cryst
- Original Message -
From: "Nave, C (Colin)" <[EMAIL PROTECTED]>
> Several have raised the issue of radiation damage. The strategy which
> Bob mentions can make sense, ensuring fresh parts of the crystal are
> regularly brought in to the beam. I would have thought 5-10 micron beams
> were
