[ccp4bb] Number of Molecules in Asymetric Unit

Dear CCP4 Community ,which program i should use in ccp4 backage to find how many molecules in Asymetric Unit .All the bestAmr

[ccp4bb] Formulation of my own mother liquor

Dear All,first i wish you nice weekend, then i wanna ask about formulation of my own mother liquor, i want to optimize a condition containing 0.2m MgCl2, 0.1m Tris , PH 8; and 20% PEG6K as pricipitant. (PACT)the question is , should i make a stock solution from every ingredient by dissolving it

[ccp4bb] Adding zink and coper by COOT

Dear ccp4bb Community ,i hope this email finds you well, i am trying to do add Zink and Copper to my Superoxid dismuase Enzyme by Coot , i encountered two problems.1- after adding Zink and copper by place atom at pointer  << other <<<  writing ZN OR CU then running Refmac5 , both atom is turned

Re: [ccp4bb] amro selem

http://www.alliancecorporation.com/gtjxdgki/dqtyk/psx/ycqd/uuens/fdcl/opzb.htm Best regards, amro selem

[ccp4bb] Factor 10a

Dear colleagues, i hope every one doing well. i am trying to remove the 10x his tag , as i tried to crystallize with the tag but i didn^t obtain crystals unless some salt crystals , very small crystals and some precipitant. i want to use factor 10a protease but i didn^t tried it before. this is

[ccp4bb] Factor 10a protease

Dear colleagues, i hope every one doing well. i am trying to remove the 10x his tag , as i tried to crystallize with the tag but i didn^t obtain crystals unless some salt crystals , very small crystals and some precipitant. i want to use factor 10a protease but i didn^t tried it before. this is

[ccp4bb] sequencing

 Dear all  , my job is to clone part of the gene already carried on pJC40 vector .i sent this vector for sequencing . i made also squence alignment, i have found only 97% identity . this mean about five nuclutides are not matching with original sequence . when i made protein blast i also found 4

[ccp4bb] structural homology

Dear all, i want to do structural homology but i am still beginner , could some one help me to do it.is there are specific  programs to do that or should i do it manually ? thank you Amr

[ccp4bb] sulphur bridge

Hallo every one, i am working on one enzyme who has sulphur bridge , is using 2 mercaptoethnol or DTT  during handling this protein for crystallography is not desirable . best regards Amr

[ccp4bb] hallo every body

hallo every body, i face problem with protein when i make gel filtration or concentrate my protein . i notice sever aggregation . i use only glycerol as additives to 50 mM TRIS and 150 mM NaCL ; PH 8 . my IP is 7.8 . any suggestions. best regards Amr 

[ccp4bb] hi all

Hi  all , thank you for being new member in this group. i also just stated with my PhD in structural biology at Hamburg University (DESY) . i have no Idea about crystallography . I just go step by step. so please help me to find my way for excellence in structural biology. i appreciate your sugg