gt; formulation for your protein, as that can significantly alter the behaviour
> of your protein in crystallisation trials.
>
>
>
> Cheers, and may your new year’s resolution be better than 2A
>
>
>
> Janet
>
>
>
> *From:* CCP4 bulletin board *On Behalf Of *amala
&
Kindly answer this
How to calculate the molecular weight of protein samples using DLS analysis
Size distribution by intensity
The purified protein was subjected to DLS - scattering light intensity
analysis (Horiba nanoparticle analyzer sz-100) and the Z average result was
71.6nm. from this result
Dear All
I am working with HIS – tag protein in N-terminal (hexa histidine). The
protein from Prokaryotic origin cloned into pET30a+ vector and
expressed in *E.coli
*BL21 cells. The expression was good. I am trying to purify a protein using
Ni-affinity column equilibrate with Buffer A 50mM Tris pH
I am trying to purify a protein using HIS-select Ni-affinity column
(washing with 50mM Tris pH7.5, 50mM NaCl, 30mM imidazole, 3mM BME,
5%glycerol and elution with the same buffer + 250mM imidazole, 500mM Nacl).
The protein (pI= 6.04) becomes cloudy/precipitated within 15minutes of
after elution. I
