[ccp4bb] Research Fellow position at Nanyang Technological University

A postdoc position (2 years at least, extendable) is immediately available in GYG’s lab, School of Biological Sciences (SBS), Nanyang Technological University (NTU), Singapore (https://dr.ntu.edu.sg/cris/rp/rp00878/selectedPublications.html). The successful applicant will be working on protein

[ccp4bb] Se SAD phasing map becomes worse after refinement

Dear All I am sorry for the long context. I have one protein (252 AAs, 2 Met) bound to double-stranded DNA (24 bp) crystalized. I collected the Se-Met data of the crystal in C222 up to 2.8 angstrom. the space group is confirmed by running the pointless. I used the Phenix.Autosol to find the

[ccp4bb] low resolution map with unmodelled map

Sorry for the initial message. I tried to attach Matthews coefficient calculation figure but failed to do so, which resulted the message as not plain text. Below is my question, thanks. I collected one dataset and processed it to 3.6 angstrom. my protein is quite small with only 14 kDa. It is

[ccp4bb] low resolution map with unmodeled maps

Dear all I collected one dataset and processed it to 3.6 angstrom. my protein is quite small with only 14 kDa. It is estimated over ten molecules in one ASU based on Matthew coefficient calculation. However, only ten molecules can be correctly placed with good fitting. I can observe extra Fo-F

[ccp4bb] Protein is dimmer in solution, hard to build two chains in crystal structure

Hi All My protein is dimer both in protein buffer and crystallisation reservoir, which is confirmed by calibration column Supderdex 200 10/300 increase. The crystal can diffract to 3 and the space group was determined to be P212121. The solvent content and Matthews coefficient shows 1 copy,