[ccp4bb] Off-topic: ligand enrichment

to binding site, enhancing the local concentration of the ligand, thus reducing the Km of the ligand. I am wondering if anybody can give some suggestions on how to solve this problem clearly. If there is any similar case, it will be better. Thank you in advance. Best wishes, Yingjie Yingjie

Re: [ccp4bb] Twinning for P31

gt; > wrong point group, they can be very misleading.. > > > > From the information you have provided I would guess the PG is P321 but > I > > need the TRUNCATE plots to be happy about saying that; they give some > > feeling for data quality. > > Eleanor &

Re: [ccp4bb] Twinning for P31

pected value of 2 for >>> untwinned data. (1.5 for perfectly twinned data) >>> However it can be distorted by non-crystallographic translation, but you >>> dont seem to have that.. >>> Or by experimental errors and you need to inspect it in resolution >>>

[ccp4bb] Twinning for P31

Best regards, Yingjie Yingjie PENG, Ph.D. student Structural Biology Group Shanghai Institute of Biochemistry and Cell Biology (SIBCB) Shanghai Institute of Biological Sciences (SIBS) Chinese Academy of Sciences (CAS) 320 Yue Yang Road, Shanghai 200031 P. R. China 86-21-54921117 Email: [EMAIL PROTECTED]

Re: [ccp4bb] CCP4i can NOT run

p in ccp4.setup seems to be incorrect. > You should have: > setenv CCP4_MASTER/home/prog > > and then you would have: > > echo $CCP4 /home/prog/ccp4-6.0.2 > > I don´t think your problem has anything to do with tcl setup. > >Boaz > > - Origin

Re: [ccp4bb] CCP4i can NOT run

I have checked /etc/hosts, it is similar to what you say. I also tried to set it exactly the same as yours. But it did NOT work. Thanks! On Tue, Sep 30, 2008 at 10:24 PM, Ben Eisenbraun <[EMAIL PROTECTED]>wrote: > On Tue, Sep 30, 2008 at 10:48:27AM +0800, Yingjie Peng wrote: > > I

Re: [ccp4bb] CCP4i can NOT run

xecutable is called something like "tclsh8.4" then CCP4i will > not recognise it..you have to make a symbolic link to the executable, in the > same directory, as below: > > ln -s tclsh8.4 tclsh (or the other way round, check)..also make sure you > have read (and possibly execu

[ccp4bb] CCP4i can NOT run

ry much in advance. Best wishes, Yingjie Yingjie PENG, Ph.D. student Structural Biology Group Shanghai Institute of Biochemistry and Cell Biology (SIBCB) Shanghai Institute of Biological Sciences (SIBS) Chinese Academy of Sciences (CAS) 320 Yue Yang Road, Shanghai 200031 P. R. China 86-21-5492

[ccp4bb] Degradation and phasing

ce on preventing protein degradation during purification or preventing phasing during crystallization. Any comments or suggestion are welcome. Thanks in advance. Yingjie Yingjie PENG, Ph.D. student Structural Biology Group Shanghai Institute of Biochemistry and Cell Biology (S

[ccp4bb] Problem with the DLS instrument

. Many thanks in advance. Yingjie Yingjie PENG, Ph.D. student Structural Biology Group Shanghai Institute of Biochemistry and Cell Biology (SIBCB) Shanghai Institute of Biological Sciences (SIBS) Chinese Academy of Sciences (CAS) 320 Yue Yang Road, Shanghai 200031 P. R. China 86

[ccp4bb] Binding pocket volume

Dear all, Is there anyone who can tell me how to calculate the volume of substrate binding pocket in a protein structure? I want to use it to the quantify the conformational change caused by induced fitting. Thanks very much. Yingjie Peng Yingjie PENG, Ph.D. student Structural Biology Group