er your loop to prevent the crystal from drying out.
>
> Good luck!
> Herman
>
> -Original Message-
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Yi-Liang Liu
> Sent: Thursday, August 02, 2012 4:15 AM
> To: CCP4BB@JISCMAIL.A
ence. Also,
> have you shot an xtal at room temp - to see what the intrinsic diffraction
> limit is? Additive screens? If all else fails you may well need to explore a
> different expression construct.
>
> Tony.
>
> Sent from my iPhone
>
> On 1 Aug 2012, a
Hi CCP4BBers,
I have a protein crystallized with 0.2mM Mg acetate and PEG 8000 or 0.1mM
cacodylate, pH 6.5, 0.2 Mg acetate, PEG 3350. Both of the conditions gave
triangle pyramid like crystals. I brought the crystals to synchrotron using 30%
PEG 400 as cryoprotactant, the resolution was only be
Hi CCP4ers,
I know it is not quiet related to CCP4. I am now working on a protein-protein
complex system. I am wondering which kits I should try in a higher priority? I
appreciate everyone's suggestions, and maybe there are some papers I can read
first.
Lucas
Hi Everyone,
I've checked the previous posts about how to generate the difference
map from two crystals with different cell constants. I tried MAPMAN to
generate this map but I never got luck on this. It turned out to show
"Maps have different cell constants" every time. Does anyone know wh
Hi Everyone,
I have a question about the crystallization condition. Currently the
condition I use contains 0.2M lithium sulfate. However, the ligand
there seems to compete the site where sulfate binds in the active
site. Are there any good substitute to replace the Lithium sulfate in
my c
