[ccp4bb] SeMet in S2 cell

Dear All, Does anybody have experience with SetMet protein in S2 cells? What kind of reagent should I use and is anything else I should pay attention to? Thanks in advance. Best, Xianchi

[ccp4bb] Refinement of 4 domain twin data

Dear All, Does anybody know if Phenix can refine the structure with 4 domain twin like refmac? If it can, how to write the input file? Best Xianchi

[ccp4bb] thin shell Rfree set selection

Dear all, I have a dataset of C2 symmetry and 2 molecules in the ASU. I am wondering if I have to use thin shell Rfree set selection to avoid Rfree bias by NCS. And which Rfree selection method is better? Thanks in advance. Xianchi

Re: [ccp4bb] off topic question BIAcore problem

SPR assay. On Wed, Feb 20, 2013 at 12:03 PM, xianchi dong wrote: > Dear all, > > Recently I have measure a set of kinetic data of a receptor ligand > interaction using BIAcore 3000. To my surprise, the dissociation rate is > very low (~ 10e-6). During the measurement, I use a lo

[ccp4bb] off topic question BIAcore problem

am wondering if the BIAcore can measure such a low off-rate kinetic. What is the limitation of BIAcore? Any review about that? Thanks in advance. Xianchi Dong Research Fellow Children's Hospital Boston Harvard Medical School