[ccp4bb] Optimal settings for finding NCS from density

2015-07-03 Thread William Chao
Dear all, What would be the best settings to look for NCS using phenix.find_ncs_from_density on an elongated (~150A) molecule? No coordinate is available unfortunately and there should be 2 copies in a P21 space group. Many thanks in advance! William --- The Francis Crick Institute Limited is

[ccp4bb] Maximising anomalous signal in helical/line scan

2015-04-24 Thread William Chao
Dear all, Does anyone have experience in maximising anomalous signal during a helical/line scan? Most (all?) automated data collection strategies in synchrotrons seem to work on a single point of a crystal but don't give strategies for line scan. Would it be advisable to increase the recommende

[ccp4bb] Thank you everyone! - Poor experimental phases for a largish structure...

2015-02-19 Thread William Chao
Dear all, Thank you so much for all the replies and suggestions in such a short period of time since I posted my questions! I am slightly overwhelmed by the number of things that I could try in the near/medium future! One thing that I am recommended a lot is TaBr. It seems that this compound is

[ccp4bb] Poor experimental phases for a largish structure...

2015-02-18 Thread William Chao
Dear all, I am trying to phase a largish novel structure of 130 kDa with P21 (sometimes P222) space group. So far I have collected a few 3.5ish-Å 3-wavelength MAD datasets from SeMet derivatives as well as an anisomorphous 6Å Hg peak dataset (with detectable anomalous signal). As my crystal is