If your crystal diffracts well and tough enough for high redundant
data collection, no HA or using sulfur in your protein will be the
first choice. Even at Cu K-alpha. I think...
N. Watanabe
On 2009/10/27, at 3:32, james09 pruza wrote:
Dear All,
I need some suggestions regarding the SAD p
Does your system eliminate the need for cryoprotection?
Since the method removes water on the crystal before freezing, in some
cases, we can go with lower concentration of cryoprotectant, like
Paratone-N. But I usually use cryoprotectant for safety.
Also, how do you time the buffer-remova
I am not sure this is the answer for your question, but please check,
http://www.nusrc.nagoya-u.ac.jp/WatanabeLab/XtalMount/index.html
We named it as a "loopless" mounting method in the paper, but now we
leave the loop...
The vacuum cleaner I have used for the method is "my mouth". But we
h
If you will use the Nextal device, I have also modified it something
like fig.2 for checking crystals in the drop at room temperature. It
is not difficult to make, too. Please see,
http://journals.iucr.org/j/issues/2005/02/00/he5324/he5324bdy.html
However, getting the film I have used in the
