[ccp4bb] [Off-topic]: Looking for a used R-Axis IV++ detector (USA)

Dear all: If anyone is willing to give away an R-Axis IV++ detector (for free or for a reasonable price) that we could use as is or dissect for parts (especially the laser assembly) please contact Frank Whitby (fra...@biochem.utah.edu). We are willing to travel (

Re: [ccp4bb] help on preparing EM map for mask generation and molecular replacement

Hi Fei: I once had the same error message when using an EM map as an MR model. To make this work, you need to change your map's axis order from XYZ to whatever is appropriate (in my case it was ZXY) and also change the spacegroup to P1 using the CCP4 program "maputils". In the CCP4i GUI, you d

[ccp4bb] Off-topic: Carboxypeptidase

Hi All: I wish to identify and remove unstructured regions at the C-terminus of my protein for crystallization and am considering using carboxypeptidase digestions to achieve this. 1) I would like a protocol and your preferred source of commercial carboxypeptidases as well their storage co

Re: [ccp4bb] Off-topic: Fungal growth in robot trays

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Viswanathan Chandrasekaran Sent: Friday, March 08, 2013 4:24 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Off-topic: Fungal growth in robot trays Dear All: I would like some advice on getting rid of persistent fungal growth in

[ccp4bb] Off-topic: Fungal growth in robot trays

Dear All: I would like some advice on getting rid of persistent fungal growth in 96-well sitting drop crystal plates that were set up using a Phoenix robot. 24-well sitting drop trays prepared by hand don't have this problem. Washing the robot with 0.5% bleach followed by plenty of water had no e