Re: [ccp4bb] native gel

Dear Jothi, Here is an alternative. Selection of membrane protein targets for crystallization using PFO-PAGE electrophoresis. Cleverley RM, S

[ccp4bb] off topic - C-ter cleavable his tag

Hi, I am looking for a vector with a cleavable C-terminal his tag for prokaryotic protein expression. I found pET-52b but it has also got a N-terminal cleavable strep-tag which I cannot avoid. Thanks Vijay

[ccp4bb] protein degradation?

Hi, I have been trying purify a N-ter his-tagged protein over-expressed in E.coli. After purification (Ni-NTA or Co-Talon), I find two bands in SDS PAGE which are very close each other (top band in the right MW and more intense than the lower band). Western blot (for his-tag) of the gel gave signa

Re: [ccp4bb] few organophophates coordiantes

Dear Sam The HIC-Up website link is http://xray.bmc.uu.se/hicup/ Hope it will help you Vijay Kumar, SRF Protein science and Engg. Division IMTECH, Sector -39A, Chandigarh INDIA U Sam <[EMAIL PROTECTED]> wrote Hi, Thanks for the reply to my earlier