Re: [ccp4bb] Semet in non-auxotrophic strains (summary)

Vangelis -- Vangelis Christodoulou Analist C Anastassis (Tassos) Perrakis lab The Netherlands Cancer Institute (NKI) B8.038 Plesmanlaan 121, 1066 CX, Amsterdam The Netherlands tel: +31 (0)20 512 1963 fax: +31 (0)20 512 1954 Website: xtal.nki.nl/Tassos_group/ On 4 Nov 2008, at 03:27, Artem

Re: [ccp4bb] Off-topic: Native gel electrophoresis of basic proteins

Hi Ming, You can find a protocol for low pH native gel electrophoresis (as well for high pH and blue native) in the following book: Gel Electrophoresis of Proteins: A Practical Approach (Practical Approach Series) by B. D. Hames. http://www.amazon.com/Gel-Electrophoresis-Proteins-Practical-A

Re: [ccp4bb] 3D model building without CRT monitor

There is a 20-inch (20-3D2W04) display that costs around $6000. For $13000 you get a 42-inch, so I guess the 52-inch that was introduced today will cost a lot. Vangelis On Jun 5, 2008, at 7:30 PM, Juergen Bosch wrote: Interesting! Their PDF says it works with OpenGL: http://www.busin

Re: [ccp4bb] 3D model building without CRT monitor

Philips is offering an interesting solution: http://www.business-sites.philips.com/3dsolutions/products/3dscreens/index.html Vangelis On Jun 2, 2008, at 2:10 PM, Krojer,Tobias wrote: Dear all, recently some of our CRT monitors broke down and we realized that these monitors are no longer pro

Re: [ccp4bb] insect expression system

e a stable HEK293 cell line expressing the same protein (at similar levels with transient transfections) with the Flp-In system in just a couple of weeks. We also have a LIC vector which is compatible with the Flp-In system. Hope this helps, Vangelis -- Vangelis Christodoulou Analist C Anastass

Re: [ccp4bb] Removal of glycosylation sites in Picha expression construct

Hi Evette, Have you tried to mutate one glycosylation site at the time and check for expression? Often glycosylation is necessary for secretion -that's the case with my protein. Also these sites tend to have a defined glycosylation pattern which eliminates the need of deglycosylation. You c

Re: [ccp4bb] crashing-out protein eluted from Nickel column

freezing it in that buffer. Good luck, Vangelis -- Vangelis Christodoulou Analist C Anastassis (Tassos) Perrakis lab The Netherlands Cancer Institute Division of Molecular Carcinogenesis - H2 Plesmanlaan 121, 1066 CX, Amsterdam The Netherlands tel: +31 (0)20 512 1951 fax: +31 (0)20 512 1954 Website

Re: [ccp4bb] Tag, you're in! Flag, V5, etc

://nar.oxfordjournals.org/cgi/content/full/35/6/e45 Hope this helps. Vangelis -- Vangelis Christodoulou Analist C Anastassis (Tassos) Perrakis lab The Netherlands Cancer Institute Dept. of Molecular Carcinogenesis - H2 Plesmanlaan 121, 1066 CX, Amsterdam The Netherlands tel: +31 (0)20 512 1951 fax: +31 (0)20 512