Re: [ccp4bb] Calculating over all bend in a DNA

2014-10-22 Thread Valerie Pye
Hi Sasha - you can use 3DNA: http://x3dna.org/; http://w3dna.rutgers.edu/ HTH, val On 21 October 2014 20:53, Sasha Pausch wrote: > Hello CCP4bb, > > May I know how can we calculate or use any server which can calculate the > overall bend in a DNA (crystal structure)? > >

[ccp4bb] Off topic - weird fluorescence in non-modified oligos

2013-11-27 Thread Valerie Pye
Sorry for the off topic, was wondering if anyone could help us out with this: We have been using a reliable supplier for synthetic oligonucleotides. Recently, we started to order oligos that we use most often on a large scale (10-umol), always RP-HPLC purified, oligos come lyophilised as white po

Re: [ccp4bb] Off-topic: NMR and crystallography

2013-06-10 Thread Valerie Pye
Hi Theresa, I think you have to be very careful with NMR of homo-oligomers, even if they’re small proteins: the NMR model/structure (backbone only) of a small integral membrane kinase was a huge effort - http://www.ncbi.nlm.nih.gov/pubmed/19556511 but is very different from the recently published

Re: [ccp4bb] help regarding structure solution

2012-06-21 Thread Valerie Pye
Hi Sonali, You could try wide-search MR: https://portal.sbgrid.org/d/apps/wsmr/ Best of luck, val On 20 June 2012 19:13, sonali dhindwal wrote: > Dear All, > > I am working on a protein for last so many years and for which i have got > crystal now in a tray which i kept 1 years ago. It diffra