[ccp4bb] Off-topic: amphipathic polymer NV-10

amphipathic polymers (A8-35, PMALs, NAPol or any others) could be used as a substitute for NV-10? Given that these amphipols are very expensive, and we would need considerable amounts of them, I would certainly like to narrow down. I will highly appreciate any input. Thanks and regards, Uma. --Uma

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

We used to use 1% solution of dimethyl dichloro silane in toluene to siliconize glass coverslips. Soak them for 30 min, rinse well with deioinized water, and oven/air dry.Uma. --Uma Gabale, PhDResearch AssociateMolecular and Cellular Biochemistry Indiana University Bloomington On Thursday

Re: [ccp4bb] Unidentified electron density blob

as an alternate conformation, or lower occupancy. Even then that would satisfy a small part of the green density, not all. The remaining residues surrounding the density are well modeled. Thanks again, and regards,Uma. --Uma Gabale, PhDResearch AssociateMolecular and Cellular Biochemistry Indiana Unive

Re: [ccp4bb] Unknown electron density blob

Dear all,Thank you very much for your replies. It is a PEG, a "di"ethylene glycol to be precise, in most chains. Best regards,Uma. --Uma Gabale, PhDResearch AssociateMolecular and Cellular Biochemistry Indiana University Bloomington #yiv6125321912 #yiv6125321912 -- _filtered #yiv