[ccp4bb] Spring School 2015: Structural Systems Biology – From Molecules to Organisms

Hello everyone A Symposium with a Spring School is being organized here in Hamburg: http://www.cssb-hamburg.de see Events Topic of the Symposium is Systems biology and Infection biology Topics in the Spring School are various Methods and Techniques, especially the EM / Single Particle. I am invol

[ccp4bb] Cyro cooling system

Hello We are planning to buy a small cryo cooling unit for protein crystal cooling which can manage 100 K temp. we are limited with space and only small units like Oxford cryosystem Desktop cooler or any of that dimensions can fit. Does anybody has some suggestions in this regard. Would be n

[ccp4bb] cryoprotection

Hello Can anyone suggest a cryoprotectant for the following crystallization condition 0.2-0.4M sodium formate ~20% PEG 3350 0-25 mM Nickel 0-100 mM Malonate Thank you with regards uday

[ccp4bb] tool for calculating average alpha helical rise per trun

Hello could any suggest some ways/tools to calculate average alpha helical rise per turn for a helical model from PDB. Thank you in advance regards uday

[ccp4bb] XRD pattern of CC

Hi I would like to know whether it is possible to get to know the number of molecules (helices) present in a coiled coil based on its XRD pattern. I only know that for coiled coil there will be a meridian arc around 5.1 A (corresponding to helical repeat) and an equatorial arc around 10 A (cor

Re: [ccp4bb] SeMet protein behaves much different with native protein..

Hi sarah I believe, you might have used reducing agent in your SeMet-labeled protein sample. if avoiding reducing agent is not a problem to your protein (ignoring Selenium), try to purify/crystallize your protein in the absence of reducing agent. if u want to try this use all degassed buffers