Re: [ccp4bb] Peptide solubility issues

25 uM (containing up to 5 % solvent), from a stock of 50-100 % solvent at 1 mM.. Btw I forgot.. for SPR (depends on the system, and especially for a 12mer), some detergents and even a minor DMSO/ethanol can affect the measurements drastically. Best wishes toufic el arnaout On Wed, Mar 5, 2014 at 12:

Re: [ccp4bb] delete subject

ing to publish their already solved structure, > or biology group that could use insights gathered by your structure > information for a publication that might limit your own ability to publish. > Fortunately, for Tom his structure sounds like it is only important to a > pretty specific scientific question that many folks might not be working on > exactly. > > Scott > > > -- ** Toufic El Arnaout Trinity Biomedical Science Institute (TCD) 152-160 Pearse Street, Dublin 2 **

Re: [ccp4bb] Oligomerization state

, might need fluorescence background optimization, also trying different hydrophobic compounds/detergents etc ************** Toufic El Arnaout Trinity Biomedical Science Institute (TCD) 152-160 Pearse

Re: [ccp4bb] Thrombin cleavage of membrane protein with fusion tag

ard Medical School > Research Associate, Brigham and Women's Hospital > Visiting Research Scholar, Brandeis University > > -- ** Toufic El Arnaout Trinity Biomedical Science Institute (TCD) 152-160 Pearse Street, Dublin 2 Tel.: +353 85 83 40 157 **

Re: [ccp4bb] CCP4MG and python.exe

Hi Rex, Maybe it is related to a 3rd party package then.. Are you using Windows? Check C++ (https://www.microsoft.com/en-us/download/details.aspx?id=) or mySQL (https://www.mysql.com/downloads/) Try to reinstall these, probably might help, but be sure about the 64 or 32 bit version. toufic O

Re: [ccp4bb] usefulness of cacodylate?

..)... you will maybe need many crystals to collect a full dataset.. for a small crystal you might not see the peak of Selenium in a scan (covered because of the caco. use). Regards toufic el arnaout On Fri, Nov 9, 2012 at 12:26 PM, Frank von Delft < frank.vonde...@sgc.ox.ac.uk>

Re: [ccp4bb] Faculty positions at Molecular Biophysics Unit, IISc, Bangalore

There is a big difference between reading "does not discriminate on the basis of race, color, religion, sex, age, sexual orientation, marital status, national origin, disability, or status as a disabled, Vietnam-era, or other eligible veteran" and "below 35 years". Then regarding Prof. Chitta's rep

Re: [ccp4bb] inflluence of pH for crystallization on protein 3-D structure

. I can't remember the details but a virus protein crystal structure was once crystallized at both pH 4 and pH 7 which gave totally unrelated structures. Functional studies are important before the interpretation of the structure. Regards Toufic El Arnaout Membrane Structural and Functional Bi

[ccp4bb] Nobel Prize 2012

Congratulations to Robert Lefkowitz and Brian Kobilka: Nobel Prize in Chemistry, 2012! Congrats to the protein structure community. toufic el arnaout

Re: [ccp4bb] [OT]: Purification in ArcticExpress

Toufic El Arnaout Membrane Structural and Functional Biology Group Trinity College Dublin On Mon, Sep 24, 2012 at 3:38 PM, Katherine Sippel < katherine.sip...@gmail.com> wrote: > Hi All, > > I've recently swapped over to expression in the ArcticExpress(DE3) cells > for a

Re: [ccp4bb] the lysozyme of membrane proteins?

to trials. Regards Toufic El Arnaout Membrane Structural and Functional Biology Group Trinity College Dublin On Tue, Sep 11, 2012 at 10:18 PM, Ho Leung Ng wrote: > Hello, > > I am developing an undergraduate biochemistry lab class and > would like to incorporate experiments with m