Tianyu,
You might try UV absorbance at 205 nM, where the protein backbone is the
absorbing moiety -- not aromatic side chains.
The Clore lab web site hosts a calculator that will estimate an E(205)
from an amino acid sequence (http://spin.niddk.nih.gov/clore/ , link on
the left hand side of
Dear all,
If anyone on this list has experience with the Fisher Scientific Isotemp
(model: 11-679-25CN) as a crystallization incubator, I would appreciate
any comments or recommendations. Many thanks in advance.
Sincerely,
thomas.
