Re: [ccp4bb] [EXT] [ccp4bb] Crystallizing a tough target

you the best of luck, Tao-Hsin Chang > On Feb 5, 2024, at 10:05 AM, kavyashreem wrote: > > Dear all, > > Thank you all for your valuable experiences, inputs and references!! I shall > try them and hope for some good news! Its good to know there are so many > examples of cr

Re: [ccp4bb] Fragile Crystals

Hi Morgan Elizabeth, Just following up on Vaheh’s suggestion, you can also add 2-4% of cryoprotectant, which includes your mother liquor (and your protein if crystals have dissolved), into the crystallization drop daily or weekly after crystals appear. Repeat this step until reaching the desire

Re: [ccp4bb] criteria to set resolution limit

Hi Farhan, It looks like that your diffraction data has an anisotropic issue and it leads to the issues of resolution limit, intensity, and completeness. Check The STARANISO Server (https://staraniso.globalphasing.org/cgi-bin/staraniso.cgi

Re: [ccp4bb] crystallizing fusion proteins

C (not ideal, but simple). Hope this helps. Best wishes, Tao-Hsin Tao-Hsin Chang, DPhil Research Specialist Howard Hughes Medical Institute > On Mar 15, 2021, at 5:07 AM, Schreuder, Herman /DE > wrote: > > Dear Bulletin Board, > Sorry for the slightly off-topic question, but we

Re: [ccp4bb] Stabilizing Mitegen reusable bases/mounts

Hi Patrick, I have met the same issue and that is very bad for any micro-focus beam and small crystals. I have talked with Mitegen and they are developing a new type of reusable base having an improvement for this issue. You may get some update from them. But, I do not use the reusable bases an

Re: [ccp4bb] Soaking to Remove Bound Ligands from Crystals

Dear Critton We have done a case, which was to remove the ligand A from the crystal in complex with ligand B and metal ions. Please see the link of this case below (Methods). http://www.ncbi.nlm.nih.gov/pubmed/20139160 Cheers, Tao-Hsin