[ccp4bb] Reg. ITC machine

s and Cons) on the above machines, which would help us in the decision process. Greatly appreciate your response. Thank you for your valuable time. Best, Sivaraman Padavattan To unsubscribe from the CCP4BB list, clic

Re: [ccp4bb] Fab purification and crystallization

Mono-S column to separate the Fab isoforms from (Fab)2 and undigested IgG. The purified Fab mixed with antigen 1:1.2 molar ratio and used the gel filtration to separate the complex from unbound antigen. Hope this will help you Cheers Sivaraman Padavattan On Fri, Sep 10, 2010 at 8:29 AM

[ccp4bb] Reg Protein purification

protein free of nucleic acid contamination. Thanks in advance, Sivaraman Padavattan

[ccp4bb] Reg. Protein purification

? Thanks in advance, Sivaraman Padavattan

[ccp4bb] Enzyme inhibitor

Dear all, I am performing an assay to screen for the inhibitor molecules. One of our compound shows inhibition at 100 μM and activation at 500μM conc. What could be the possible reason for the compound work differently in two different conc. Thanks Sivaraman