I'm still using passive, interleaved 3D, Zalman-style... Hopefully coot etc
will retain support for that...!
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We have seen a similar thing once, where we obtained proteins crystals simply
by dehydration of the drop:
Sharpe, M. L., Baker, E. N., and Lott, J. S. (2005) Crystallization of a
protein using dehydration without a precipitant. Acta Crystallogr Sect F Struct
Biol Cryst Commun 61, 565–568.
They
Thanks to everyone who responded, on and off the list. I thought I'd post a
quick summary.
To clarify my question slightly (if belatedly) I'm specifically looking for a
passive 3D solution to plug into my MacBook Pro whilst on sabbatical in the
USA. I'll be using it for a mixture of 2D and 3D v
A rather US-centric question on passive 3D monitors...
I'm just getting set up in the US, and I'm surprised on how few passive 3D
monitors seem to be around - many models seem listed as 'out of stock' when
looking in the usual places (Amazon, NewEgg, BestBuys, Walmart etc.)
The best deal I have
Yep - works fine on Coot and Chimera. Others may correct me, but I don't think
Schrodinger have re-instated support in PyMol?
I use it daily - the Zalman is the primary monitor on my desk. The great
advantage of this type of monitor (IMHO) is that it is hardware independent.
Just to add my 2c worth...
The department here has a couple of nanodrops as a shared facility, one for
DNA/RNA and one for protein. It has been noticeable that over time people has
been getting decreased reliability of measurements on the latter machine cf
cuvette measurements, presumably due t
As with all these things, YMMV.
In our experience, chaperone co-expression can help not at all, can produce
more folded protein or can produce more soluble protein, with co-purifying
chaperone. With GroEL/S at least, chaperone release is easy (add ATP) and
sometimes results in the liberation of
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I have the previous model with NVIDIA GeForce 9400M and it's been just fine -
on the desktop I use a Zalman stereo and it's great.
Or you could just bug me to find the PNGaseF expression plasmid that I should
have in the freezer somewhere!
LOO T., PATCHETT M. L., NORRIS G. E. & LOTT J. S. “Using Secretion to Solve a
Solubility Problem: High-yield Expression in E. coli and Purification of the
Bacterial Glycoamidase PNGase F
In my experience, DALI can be better than SSM at detecting distant
structural similarities. You might also want to try CE. To decide if a fold
is 'new' rather than 'old but decorated' often boils down to a somewhat
subjective call, but asking Alexei Murzin is as good a way as any to decide :-)
For
>For gel-shift assay, do people normally use a special gel tank for heavy
>metal work?
We used to use a Pharmacia Phast system, as this is bufferless and made it
very easy to contain heavy metal contamination. Ours caught fire and died a
couple of years ago, and I have yet to find a good cheap r
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