Brian,
We use a PlateLoc Thermal Microplate sealer from Agilent. Works very
well, pricey though ...
Cheers,
Carsten
> -Original Message-
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Brian Mark
> Sent: Tuesday, June 14, 2011 5:19 PM
> To: CCP4BB@JISCM
Eric, Takaaki:
I just remembered that we ran into the same problem. If you are using Linux try
launching 'nvidia-settings' and disable GPU scaling. That helped with some of
our monitors, which exhibited the same problem. Not sure if that would be
applicable to Windows though.
HTH
Carsten
Andreas,
I can only second what Chris was saying. We have the same setup (FX3800
with separate riser for stereo connection) used in all of our machines
and it works very well. In terms of monitor I would recommend to spend a
bit more money and go with an Alienware or Asus 3D LCD, both are running
Eric,
if you want to use stereo on a notebook you have very limited options. I have
stereo running using a Lenovo T61p with a Nvidia Quadro FX570M under XP, not
tried Linux yet. Officially this card is not even supported by Nvidia for 3D
application, but works anyway in conjunction with a dock
Hi Xiaopeng,
In addition to what has been suggested:
Make sure to understand that your 'inhibitors' are really inhibitors and
not just general destabilizers of proteins. A binding assay like ITC or
Thermofluor like assay is useful to establish that your inhibitors are
actually binding to the prot
Tim,
I'm shooting from the hip here, but I think the problem is that cctbx
comes with its own python, which you would need to run both pymol and
numpy under. According to Ralf it is at least not trivial to utilize the
system python to run cctbx. So I think you need to compile/install pymol
against
Paul,
couple things come to mind:
-make sure your substrate is actually a binder and not an (unspecific)
inhibitor (ITC, Thermofluor, Biacore)
-soak longer and at higher concentration. (3mM concentration from 100mM
stock in DMSO for a week or more)
-Is there evidence that your protein needs to
Jane,
you could use ksDSSP, which is an OpenSource implementation of DSSP.
Requires some compiling though and I am not sure if OSX is supported.
You also need to compile the PDB record I/O libraries.
http://www.cgl.ucsf.edu/Overview/software.html
Cheers,
Carsten
> -Original Mess
Chris,
For the laser-etched glass blocks have a look at:
http://www.bathsheba.com/
I have a couple of them and they look very good.
Cheers,
Carsten
> -Original Message-
> From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
> Christopher Rife
> Sent: Tuesday,
http://www.mitegen.com/
Cheers
Carsten
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk]on Behalf Of cedric
bauvois
Sent: Friday, January 16, 2009 9:27 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] cryoloops for X-ray data collection from protein crys
e "classical" ones.
Bert
-Original Message-
From: CCP4 bulletin board on behalf of Schubert, Carsten [PRDUS]
Sent: Thu 10/9/2008 9:15 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] foam dewar usage ?
Hmmm, that's funny. Our experience was quite the opposite. The first time we
used
Hmmm, that's funny. Our experience was quite the opposite. The first time we
used one of the darker small dewars we ended up with a lot of burr in the LN2.
Those got picked up by the cryo and we ended up with lilac particles embedded
inside the cryo. You can imagine the reaction when these parti
Christina,
phenix.elbow will generate the CONECT records for you.
HTH
Carsten
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Behalf Of Christina Bourne
Sent: Thursday, October 02, 2008 12:33 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] CONECT records
David,
check if your personal environment (login scripts, paths, etc...) is the same
on your home machine and the other machines. Looks as if there might be a
difference. What does the coot console say, any error messages or hints?
HTH
Carsten
> -Original Message-
> From: CCP
Simon,
solubilize your ligand in DMSO so it is maximally concentrated, 100mM works
fine. Add enough compound to achieve 2-3 fold excess to your protein, mix and
set up. Make sure your final DMSO concentration is ~3%, otherwise chances are
you might harm your protein. If you cannot achieve a hig
Paul:
that is a problem with the NVIDIA hardware. In some old release notes 2 years
ago they spoke
of some instabilities of the GPU with regards to stereo. This happens more
under linux than under windows, which points to a driver issue. To my knowledge
there is not much you can do.
May be so
Aha Dr. Palm
Tough to generalize this, but if the ligand is weak or the site only
partially occupied you need to refine almost to completion. Since you seem
to be doing something akin to fragment screening I can only pass on my
experience. I had plenty of cases where the Rfree was ~35 after a
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