Hi David Mueller,
In Physical Chemistry Van der Waals interacions are defined as all type of
forces between molecules (or parts of them) excluding covalent bonds and
electrostatic interactions. So, you are right that the most common forces
included into Van Der Waals are: interaction between perm
Dear Gregory and Darren,
Thank you for your answers. They have been very useful.
2012/1/24 Gregory Verdon
>
> it possible that the protein is toxic (even when slightly expressed from
> your possibly leaky pET vector), so that e.coli select for mutations that
> kill expression of your recombinant
Dear everyone,
I am trying to clone a viral protein in the E. Coli BSJ strain and i am
having some problems.
I start from the viral RNA carrying out a reverse transcription and PCR
(RT-PCR) to obtain the protein cDNA. When I sequence this cDNA to check for
mutations, there are no mutations. So th
