I hope this isn't too much of a thread hijack, but i also wanted to add a
question to Randy's : How big a protein can one express in insect cells?
I've read in a few places that it's not size limited but i wonder if
that's borne out in practice.
On 9 May 2013 16:05, Randy Watson wrote:
> Hi a
Dear all - is there a rule of thumb for favourable values of Kd, kon and
koff of protein-protein or protein-dna complexes for protein
crystallisation? Are these measurements useful in crystallisation, or
should one just put it down a gel filtration column, hope for a complex and
not worry? If an