Re: [ccp4bb] dimeric region

Hi Rohit, A nice tool I came across recently, other than PISA, was EPPIC: http://eppic-web.org/ewui/ . This tool analyses the interface in a similar way to PISA, looking at size and gives a per-residue percentage buried surface area. It also does an evolutionary analysis to determine how conserve

Re: [ccp4bb] OFF TOPIC: Cooling system for columns

Hi Chiara, We also have an Avant at room temperature. My recommendation would be to forgo using a water bath. Instead you can achieve a prep near enough to 4C by doing 2 things: Firstly, keep your columns stored in the cold room or a fridge to pre-chill them before use. Secondly, make your buffers

Re: [ccp4bb] Problems in purification

This short paper in Nature Methods is probably worth a read and may help with your Ni-NTA issues: http://www.nature.com/nmeth/journal/v6/n7/full/nmeth0709-477.html Nature Methods 6, 477 - 478 (2009) doi:10.1038/nmeth0709-477 Enabling IMAC purification of low abundance recombinant proteins from E.

Re: [ccp4bb] local copy of wiki

(just forwarding this to the list...) > Dear Tim, > > I think it may be possible, though rather long-winded to do. First of all, > you'll need a working installation of mediawiki. My suggestion to get this > would be to download one of the self-contained installations available from > bitnami: ht

Re: [ccp4bb] Alignment software

Hi there, I just got this working a couple of days ago under Mac OS X 10.6.3. I manually compiled and installed Perl-Tk from CPAN. Steps taken: 1. Download and ungzip / untar: http://search.cpan.org/CPAN/authors/id/S/SR/SREZIC/Tk-804.028_501.tar.gz(the newer -503 version didn't work) 2. Change in

Re: [ccp4bb] OT: VectorNTI alternatives - 4 Mac?

b] OT: VectorNTI alternatives - 4 Mac? From: Mark Collins To: CCP4BB@JISCMAIL.AC.UK Date: Wed Jan 28 15:02:17 2009 Hi Anybody have suggestions for Mac OsX alternatives? Thanks in advance, Mark -- Roger Dodd PhD The Institute of Cancer Research Chester Beatty Laboratories 237 Fulham Road London SW

Re: [ccp4bb] Create a dual boot windows-linux machine

? Thanks in advance, Xie -- Roger Dodd PhD The Institute of Cancer Research Chester Beatty Laboratories 237 Fulham Road London SW3 6JB UK The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company Limited by Guarantee, Registered in England under Company No. 534147 with its