Re: [ccp4bb] Incorrect chiral volumes error during real space refinement

2017-02-28 Thread Ries, Anne
nement target. You may need to reduce the matrix weights on the map (Extensions -> Refine -> Set Refinement Options). Best, Arjen On 28 February 2017 at 15:40, Ries, Anne mailto:anne.r...@helsinki.fi>> wrote: Hi, I am working with a cryo EM map at 4.2 A and would like to de novo build

[ccp4bb] Incorrect chiral volumes error during real space refinement

2017-02-28 Thread Ries, Anne
Hi, I am working with a cryo EM map at 4.2 A and would like to de novo build a model into the map. As our map is initially calculated in .mrc format, we converted it into .mtz format. I used a poly-Ala helix .pdb file as a model to begin with. I opened the .pdb of the helix and the .mtz map in