Re: [ccp4bb] A helix with leucine repeats

, Michael R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 513 Biochemistry Bldg. Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-

Re: [ccp4bb] Differences in a homodimer protein

. Bottom line is to be cautious and skeptical with any hypothesis you want to test about this water. Cheers, Michael R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan S

Re: [ccp4bb] ccp4 website not secure

/ <https://www.ccp4.ac.uk/ccp4online/>, which requires logins, but ccp4 is http://www.ccp4.ac.uk/ <http://www.ccp4.ac.uk/> still. I had it mostly when connecting from home. Michael ******** R. Michael Garavito, Ph.D.

Re: [ccp4bb] Help needed finding hit condition

aps at 1 M) was pH 8, but when diluted down with the other components to make the 150 mM concentration for the screen mixture, the pH can be significantly different. The dilution of Tris would drop the pH. Cheers, Michael ********

Re: [ccp4bb] Can DDM give egregious false positives when using it to crystallize membrane proteins?

chael **** R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-9334

Re: [ccp4bb] help needed to interpret a SRF

ographic b/b* axis and the second peak in the X-Z plane. These could be weak NCS two-folds. How many NCS systems do you suspect in the ASU? Cheers and good luck, Michael ************ R. Michael Garavito, Ph.D. Professor of Biochemistry &

Re: [ccp4bb] help needed to interpret a SRF

xis (North-South or y-axis).Cheers,Michael EhaAc_kappa180.pdf Description: Adobe PDF document ********R. Michael Garavito, Ph.D.Professor of Biochemistry & Molecular Biology603 Wilson Rd., Rm. 513   Michigan State University      East Lan

Re: [ccp4bb] mystery disappearance of CCP4 website(s)

I checked again after Peter’s email and they are reachable. WTF May they did pay, as Bernhard suggests. Michael R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan S

Re: [ccp4bb] mystery disappearance of CCP4 website(s)

R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-9334Email: rmgarav...@gmail.com

Re: [ccp4bb] Off topic instrumentation

. So you needed that whole setup to puncture the tube and drain it into the fraction collector to get fractions from highest to lowest density. Clunky, but it works. Do you have that? Michael R. Michael Garavito, Ph.D. Professor

Re: [ccp4bb] Protein Purification

R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-9334Email: rmg

Re: [ccp4bb] Density for an unknown ligand

R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-

Re: [ccp4bb] Color of heme containing Xtals

better, more information is needed. Cheers, Michael * R. Michael Garavito, Ph.D. Professor of Biochemistry Michigan State University East Lansing, MI 48824-1319 517-355-9724 (Office) 517-353-9334 (Fax) [EMAIL PROTECTED