Re: [ccp4bb] RMSD per residue graph for multiple aligned PDB files

from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/poli

Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

exe?SUBED1=CCP4BB&A=1 <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> ------------ -- Prof. Dr. Arne Skerra Lehrstuhl f. Biologische Chemie | Technische Universitaet Muenchen Emil-Erlenmeyer-Fo

Re: [ccp4bb] dimeric tag to induce the homodimerization of protein

--- To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ------------ To unsubscribe from the CCP4BB list, click the following link:

Re: [ccp4bb] Plasmid for cloning scFV into Fab format and Bacterial expression for crystallography

l expression, where I can just clone my scFv and it would be > ready for Fab expression (probably periplasmic or secreted in supernatant) > > If commercial plasmids are not available, could you suggest where I could > request them from? > > Thanks in advance, > Ivan Prof. D

Re: [ccp4bb] help with strange density map

eptide deformylase and fMAS > Crystallization condition: 2M Sodium acetate, 0.1 M HEPES 7.5 and 0.05M CdSO4 > > Thank you > Prof. Dr. Arne Skerra Lehrstuhl f. Biologische Chemie | Technische Universitaet Muenchen Emil-Erlenmeyer-Forum 5 | 85350 Freising-Weihenstephan | Germany Phone: +49 (0

Re: [ccp4bb] Low 280 absorbance imidazole?

> k.-k. Hofkristallamt > Vista, CA 92084 > 001 (925) 209-7429 > b...@ruppweb.org > b...@hofkristallamt.org > http://www.ruppweb.org/ > ------- Prof. Dr. Arne Skerra Lehrstuhl f. Biologische Chemie | Technische Universitaet M

Re: [ccp4bb] Metal won't strip from IMAC

ridiculous number of cysteines (10 in 416 residues > total). There is 1 mM BME and 1 mM MgCl2 in all of the buffers. > > Thanks, > > Katherine + Prof. Dr. Arne Skerra + Lehrstuhl f. Biologische Chemie + Technische Universitaet Muenchen Emil-Erlenmeyer-Forum 5 + 85350 Freising-Weihenstephan + Germany Phone: +49 (0)8161 71-4351 + Fax: -4352 http://www.wzw.tum.de/bc + eMail: ske...@tum.de

Re: [ccp4bb] Off topic - Streptactin Column

lumn and I think lately that I have been > seeing decreased binding. > > Thanks in advanced. + Prof. Dr. Arne Skerra + Lehrstuhl f. Biologische Chemie + Technische Universitaet Muenchen Emil-Erlenmeyer-Forum 5 + 85350 Freising-Weihenstephan + Germany Phone: +49 (0)8161 71

Re: [ccp4bb] Fab fragments and their stability

r -80. thanks a lot > Jan -- ---- Prof. Dr. Arne Skerra ske...@wzw.tum.de Lehrstuhl f. Biologische Chemie Phone: +49 (0)8161 71-4351 Technische Universitaet Muenchen

Re: [ccp4bb] Colored proteins :)

w out there somewhere but so far I've not found it. Thank you, Artem -- -------- Prof. Dr. Arne Skerra ske...@wzw.tum.de Lehrstuhl f. Biologische Chemie Phone: +49 (0)8161 71-4351

Re: [ccp4bb] Potential N-terminal cyclization cont.

N-term is flexible enough to accomodate the amidine then a crosslink by this mechanism is at least conceivable. I will submit a sample to mass-spec analysis to verify the presence of an additional carbon atom. Thanks for the advice. Gerwald Prof. Dr. Arne Skerra wrote: Dear Colleagues, Th

Re: [ccp4bb] Potential N-terminal cyclization - 2nd attempt with formula attached

stions would be highly welcome. Gerwald -- ------------ Prof. Dr. Arne Skerra [EMAIL PROTECTED] Lehrstuhl f. Biologische Chemie Phone: +49 (0)8161 71-4351 Technische Universita

Re: [ccp4bb] Potential N-terminal cyclization

uld be highly welcome. Gerwald -- ------------ Prof. Dr. Arne Skerra [EMAIL PROTECTED] Lehrstuhl f. Biologische Chemie Phone: +49 (0)8161 71-4351 Technische Universitaet MuenchenFax: -4352 85350

Re: [ccp4bb] 3D Glasses

4 (0)1223 252835 web: www.mrc-dunn.cam.ac.uk email: [EMAIL PROTECTED] -- ---- Prof. Dr. Arne Skerra [EMAIL PROTECTED] Lehrstuhl f. Biologische Chemie Phone: +49 (0)8161 71-4351 T

[ccp4bb] Job offer: Postdoctoral Position at TU Munich

Cluster of Excellence 'Munich Center for integrated Protein Science - CIPSM'. Applications including CV and two letters of recommendation or addresses of references should be sent to: Prof. Dr. Arne Skerra, Lehrstuhl f. Biologische Chemie, TU Muenchen, 85350 Freising-Weihenstephan, Germ

Re: [ccp4bb] Strep-tag II versus His-tag

nes Blvd, Dallas, TX 75390-9063 -- ------------ Prof. Dr. Arne Skerra [EMAIL PROTECTED] Lehrstuhl f. Biologische Chemie Phone: +49 (0)8161 71-4351 Technische Universitaet MuenchenFax: -4352 85350

[ccp4bb] Job offer: Postdoctoral Scientist in Protein Crystallography

Cluster of Excellence 'Munich Center for integrated Protein Science - CIPSM'. Applications including CV and two letters of recommendation or addresses of references should be sent to: Prof. Dr. Arne Skerra, Lehrstuhl f. Biologische Chemie, TU Muenchen, 85350 Freising-Weihenstephan, Germ

[ccp4bb] Job offer: Postdoctoral Scientist in X-ray structural analysis of plant proteins

PROTECTED]; http://www.wzw.tum.de/bc -- Prof. Dr. Arne Skerra [EMAIL PROTECTED] Lehrstuhl f. Biologische Chemie Phone: +49 (0)8161 71-4351 Technische Universitaet Muenchen