Dear all,
We have two data sets of a protein with the following parameters:
1. Space group P212121 a=61.0, b=100.34, c=133.23 No. of molecules in ASU -
2
2. Space group P212121 a=58.33, b=62.86, c=109.45 No. of molecules in ASU -
1
Can we use them as two different structures?
Regards,
Prerana
#
s) can change crystal
> morphology. You could also re-screen for new conditions either using matrix
> micro seeding, or change the protein buffer. Perhaps adding a ligand or a
> component from your current crystallisation conditions to your protein
> stock?
>
> HTH,
>
> Dave
&
Dear all,
I am working on a 40kDa protein. The size and diffraction pattern of the
protein crystal seems to be fine but I am getting poor resolution (3.0 Å).
How can I increase the resolution?
Regards,
Prerana
Hi Vicky,
I tried to change the ratio of paraffin:silicon oil to 1:2, but still the
protein precipitated. So I tried to do it other way round, I kept
paraffin:silicon oil ratio as 2:1 and so far it has not precipitated. Apart
from that, I separately used the two oils.In silicon oil there was
immed
Hi, Sorry for asking an off-topic question,
I have recently purified a protein having a molecular weight of 40kDa and
concentration of the protein was 8mg/ml. When I tried to set the protein
for crystallisation using micobatch method, the protein started
precipitating in most of the buffer conditi
