EUbOPEN projects
with partners in academia and the biomedical industry worldwide.
This position is jointly supervised by Maurice Michel and Opher Gileadi.
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Adding to the excellent advice offered by others:
1. Try to purify a unique oligomeric state: mixtures of homodimers,
heterodimers etc are less likely to crystallize. Collect a narrow peak off a
size exclusion column.
2. Try adding mono- or dinucleotides during crystallization, with or without
d
A highly motivated, skilled and experienced post-doctoral research fellow in
protein production/structural biology is needed to work at SGC-Karolinska in
our IMI (EU and Pharma) funded EUbOPEN project. You will be part of a
collaborative research environment, driving the characterization of huma
We are seeking highly motivated postdoc to investigate new targets for
treatment of Alzheimer's disease. You will be joining the Structural Biology
Core of an international, NIH-funded consortium (TREAT-AD,
https://treatad.org/emory-sage-sgc/), contributing to testing new therapeutic
hypotheses
Exiting postdoc positions for structural biologists to study new trug targets
for Alzheimers and other neurodegenerative disease. Part of a large
international consortium funded by the NIH, you will study the structure and
function of diverse proteins, working in close cooperation with medicinal
A postdoctoral position in the Alzheimer's drug discovery institute, University
of Oxford. The closing date is 12 noon on Thursday 26th July 2018
https://www.ndm.ox.ac.uk/current-job-vacancies/vacancy/135677-Postdoctoral-Scientist-Protein-Production-for-Dementia
We have an exciting opportunity for a Postdoctoral Research Scientist at the
Structural Genomics Consortium in Oxfrod. You will join a collaborative project
supported by Cancer Research UK; you will perform structural analysis of the
MBL proteins (SNM1A, SNM1B/apollo and SNM1C/artemis), in compl
Postdoctoral Scientist - Structural Biology (2 posts)
SGC, Nuffield Department of Medicine (NDM), Old Road Campus Research Building,
Roosevelt Drive, Headington, Oxford OX3 7DQ
Grade 7: Salary in the range £31,604 - £33,518 p.a.
For full details and application links see:
http://www.thesgc.org/c
In addition to the previous suggestions:
With very small gels, the sample composition and depth (in the well) have a
strong effect on the resolution.
Rinse the wells with TBE buffer just before loading, as urea from the gel
diffuses into the well and may prevent the sample from settling at the b
FP is the ratio between two fluorescence measurements; if the fluorescence
signal is too low, you will still get a ratio but it will be essentially noise.
Try to perform the measurements at 10-50 nM DNA. If your binding affinity is in
the low nM range, you may have to use other methods to measur
6th-14th of July, 2017 in Harwell (near Diamond/Oxford).
Registration deadline 15th of March.
http://meetings.embo.org/event/17-protein-production
Dear Careina,
First: make sure your solution is completely free of competing primary amines,
e.g. tris buffer. Phosphate buffers or HEPES should be used; if you need buffer
exchange, do it by gel filtration or extensive dialysis (2-3 changes), not
concentration-dilution.
Second: Try to use BS3,
"reverse translation" from protein to nucleotide does not yield a unique
sequence. Your best bet is to use the BLAST feature that searches your protein
sequence against the entire genbank DNA database translated into protein:
http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi?PROGRAM=tblastn&PAGE_TYPE=
Hi Appu,
Adding the extinction coefficients of the nucleotides is a reasonable
approximation; a somewhat more rigorous approximation takes into account
nearest neighbor frequencies; see, for example, some commercial web sites such
as http://www.operon.com/products/custom_oligos/calculations.asp
Hi Ashok,
You say your DNA is palindromic - have you looked at the possibility that the
longer DNAs are actually folded on themselves rather than duplexes of two
separete oligos? Depending on the location of the centre of your palindrome and
on interactions with adjacent molecules, this could
Hi Theresa,
To add to Anat's comments: Although the AUG codon for the first methionine and
all other methionines in a protein coding sequence look the same, they are read
in a very different way by the ribosomal machinery. The first AUG is recognized
by the initiation complex, which includes th
Hi,
We had a massive phage infection at the SGC in 2004; all our washing and
sterilization efforts could only solve the problem temporarily. I then
recovered a phage-resistant subclone of BL21(DE3), and prepared derivative
strains with pRARE2 (the tRNA plasmid in Rosetta2) or other plasmids. We
We are seeking a postdoctoral scientist to join the group of Opher Gileadi,
studying DNA repair and the structural basis of human genetic disease.
This position offers a unique opportunity for a structural biologist. You will
drive several projects from gene to structure, aimed at understanding
It makes sense to use a fixed ratio of resuspension buffer to cell weight; we
weigh the pellets after centrifugation, then suspend in at least 4-5 volumes
(ml/gr) of buffer.
Molsoft has a free application:
http://www.molsoft.com/iMolview.html
Thanks all for the comments. I was thinking of crosslinking a protein that
hasn't crystallized. Cystein engineering seems a good idea but depends on the
availability of a good model. We'll be trying mild crosslinking using
bifunctional reagents of various lengths (I suspect glutaraldehyde will n
Hi all
Does anyone have experience or insights on intramolecular crosslinking of
proteins before crystallization? The idea is to lock a felxible (multidomain)
protein into a restricted conformation.
Thanks,
Opher
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