Anindito,
First off, I have never tried this, but here are a few initial thoughts on how
I might approach this. But first a question, how tight is the dimer form? if
you mixed dimers with tags and dimers without tags, would you get any exchange?
If so, you might be able to use this to your
Pascal,
Not personally observed this myself, but just wondering, as a control, have you
tried setting trays with just the buffer+detergent (no PDC)? And if so, do you
see the same behavior or not? Also, do you see phase separation in all drops
or just a majority? Do the drops that exhibit th
Iris,
Having tackled this question myself recently, I was unable to find any software
to do this analysis for me. So, unless someone has personal scripts or
software that isn't openly available or something that I may have missed, I
think you will have to do the analysis manually. While the a