Re: [ccp4bb] Insertion of a Tag protein in of the molecules of a Dimer protein complex

2013-08-27 Thread Noinaj, Nicholas (NIH/NIDDK) [F]
Anindito, First off, I have never tried this, but here are a few initial thoughts on how I might approach this. But first a question, how tight is the dimer form? if you mixed dimers with tags and dimers without tags, would you get any exchange? If so, you might be able to use this to your

Re: [ccp4bb] membrane protein and phase separation

2013-08-01 Thread Noinaj, Nicholas (NIH/NIDDK) [F]
Pascal, Not personally observed this myself, but just wondering, as a control, have you tried setting trays with just the buffer+detergent (no PDC)? And if so, do you see the same behavior or not? Also, do you see phase separation in all drops or just a majority? Do the drops that exhibit th

Re: [ccp4bb] Shear number of beta-barrel proteins

2013-07-30 Thread Noinaj, Nicholas (NIH/NIDDK) [F]
Iris, Having tackled this question myself recently, I was unable to find any software to do this analysis for me. So, unless someone has personal scripts or software that isn't openly available or something that I may have missed, I think you will have to do the analysis manually. While the a