Re: [ccp4bb] challenges in structural biology

What about AI doing our job in the future? https://www.nature.com/articles/d41586-019-01357-6?utm_source=Nature+Briefing&utm_campaign=4c1d57fdf3-briefing-dy-20190722&utm_medium=email&utm_term=0_c9dfd39373-4c1d57fdf3-44201949 Best Regards Nishant On Mon, 22 Jul 2019 at 11:30 PM, Sarah Bowman wro

Re: [ccp4bb] xds_par licence expired error

ibution. > > best wishes Graeme > > On 20 Apr 2018, at 09:36, Nishant Varshney mailto:nikdn > a...@gmail.com>> wrote: > > Dear All, > > I am sure I have done it before but do not remember/finding the solution > online for this now. > > My XDSGUI returns with th

[ccp4bb] xds_par licence expired error

Dear All, I am sure I have done it before but do not remember/finding the solution online for this now. My XDSGUI returns with the error of xds_par "Licence Expired on 31st Mar 2018". Before Reinstalling XDS and XDSGUI on my iMac I would like to ask the experts if there is an easy solution to

Re: [ccp4bb] the purification process of protein, used for crystallization

In continuation of this thread, what is the success rate of separating TEV and MBP tag from my desired protein after TEV cleavage using Hydrophobic/Ion exchange column? I have also read TEV is less active in a high concentration of Imidazole, so dialysis before adding TEV may work better but then r

Re: [ccp4bb] MrBUMP error

vert to string > #locale = normalize(_build_localename(locale)) > #return _setlocale(category, locale) > > I don't know if this is the most elegant solution, but it works and it > doesn't seem to break anything else. > > /Derek > > On 5 Sep 2017, at

[ccp4bb] MrBUMP error

Dear Crystallographer, I will be grateful if you help me with what may be a simple problem. While trying MrBUMP with my mtz file , I am currently getting the following error. Similar error I am getting while trying AMPLE as well. "CCP4I VERSION CCP4Interface 7.0.044 #CCP4I SCRIPT LOG mrbump #CCP4

[ccp4bb] NMR or Homology Model as a MR model

Dear Crystallographers, I am working to solve an human protein structure which has a domain sequence identity of 24% with domain of another protein. As Phaser as well as Molrep failed to give any definite solution (TFZ=3.7 from MR), I want to ask, if solution structure of another protein having d

Re: [ccp4bb] XDS questions

cumented, I am not >> sure >> aimless takes into account how XDS treats the data. I would therefore >> trust >> the step of scaling to the same author and continue with XDS_ASCII.HKL. >> >> Best, >> Tim >> >> >> On Monday, November 21,

Re: [ccp4bb] XDS questions

Dear All, Just to understand more, the XDS_ASCII.HKL file generated after running XDS contains scaled and merged reflections? Moreover, what happens exactly, if you use XDS_ASCII.HKL file in AIMLESS instead of INTEGRATE.HKL file?? I ran AIMLESS separately, one using already scaled XDS_ASCII.HKL

[ccp4bb] Regarding BMCD

Dear all, I have bee trying to access the biomolecular crystallization database BMCD for quite sometime now, no matter which route I access it through the page shows an error as the server can not be contacted. I would like to know whether its a problem only I am coming across.Moreover, is th