[ccp4bb] Question about losing reflections when refining in PHENIX

2019-10-28 Thread Nemanja Vuksanovic
en atoms 2310 macromolecules 2310 Protein residues 286 RMS(bonds) 0.010 RMS(angles) 1.01 Ramachandran favored (%) 96.40 Ramachandran allowed (%) 2.52 Ramachandran outliers (%) 1.08 Rotamer outliers (%) 0.00 Clashscore 8.47 Average B-factor 43.55 Best Regards, Nemanja Vuksanovic -- Graduate Student

[ccp4bb] Re-using 96-well crystallization plates

2019-04-10 Thread Nemanja Vuksanovic
ble. Best regards, Nemanja Vuksanovic -- Graduate Student Department of Chemistry and Biochemistry University of Wisconsin-Milwaukee To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.

[ccp4bb] Removing tartrate ions from the active site

2018-04-21 Thread Nemanja Vuksanovic
Dear All, I've recently crystallized a protein under a condition containing 1.1 M ammonium tartrate. (I was able to get a few other hits but only these crystals diffract below 2.5 A). Upon refinement, it appears that the electron density in the active site shows a mixture of tartrate and the nativ