Re: [ccp4bb] precision protease plasmid

Hello BBians, sorry for the off-topic question. I have plasmid which possesses the precision protease site to get rid of his-tag. Does anybody have precision protease plasmid? If someone has please share with me. I will be very thankful to you. On Wed, 20 Jun 2018 at 10:25, Neeraj Kr. Mishra

Re: [ccp4bb] Trump

on't think I want to be friends with anyone who approves of racism, >> misogyny and xenophobia >> >> >> >> On 9 November 2016 at 18:32, Gert Vriend >> wrote: >> >> Lets stop this discussion before it divides this list of friends just as >>

[ccp4bb] Request

Hello BBians, sorry for the off-topic question. I have plasmid which possess the precision protease site to get rid of GST tag. Does anybody have precision protease plasmid. If someone has please share with me. I will be very thankful for you. Neeraj -- = Neeraj Kr

[ccp4bb] Ser/Thr phosphatase

Hi All, I am wondering if anyone can point me towards a literature where a Ser/Thr phosphatase was expressed and purified to homogeneity which was functionally active. Excuse my ignorance as I am new to this field and need some help getting started. Thanks, neeraj

[ccp4bb] kinase purification

the ground running for me. I would also very much appreciate any helpful suggestions that anyone might have. thanks Neeraj

[ccp4bb] help with kinase purification

o the precautions / pitfalls while dealing with kinase purification. Thanks and have a great year ahead! Neeraj

[ccp4bb] citation for MOSFLM

kindly mention the same so that I can send it back to the journal which requires the same. I do appreciate your kind help with the same and apologize for this but just couldn't find the information on the web so had to resort to this. Thanks, neeraj

[ccp4bb] DNA binding protein

appreciate if someone can provide any valuable tips for the same. thanks, Neeraj -- Neeraj Kapoor TPCB Graduate Fellow Sakmar Lab/ Molecular Biology & Biochemistry The Rockefeller University 1230 York Avenue, RRB 510 New York, NY 10021 lab.1.212.327.8284:fax.7904 mobile: 917.535.2030

[ccp4bb] Off topic

Hi all, Does anyone know of a good membrane protein crystallogrphy course/ conference that would be taking place sometime early next year? thanks neeraj -- Neeraj Kapoor TPCB Graduate Fellow Sakmar Lab/ Molecular Biology & Biochemistry The Rockefeller University 1230 York Avenue, RRB

[ccp4bb] creating alternate conformations using PyMol

a small fragment of the protein while keeping everything else static. thanks for the help in advance! Neeraj -- Neeraj Kapoor TPCB Graduate Fellow Sakmar Lab/ Molecular Biology & Biochemistry The Rockefeller University 1230 York Avenue, RRB 510 New York, NY 10021 lab.1.212.327.8284:fax.

[ccp4bb] Coiled Coils

of proteins. I would be grateful for any help i can get. thanks neeraj -- Neeraj Kapoor TPCB Graduate Fellow Sakmar Lab/ Molecular Biology & Biochemistry The Rockefeller University 1230 York Avenue, RRB 510 New York, NY 10021 lab.1.212.327.8284:fax.7904 mobile: 917.535.2030

[ccp4bb] Protein binding to Zn and Ca

Hi all, I recently came across a question about an interesting idea. Does anyone know of an example of a protein binding to both Zn2+ and Ca2+ at the same time? Are there any known well studied precedents at all if any. Any help of insights would be very valuable. thanks, Neeraj

[ccp4bb] topology diagram

Hi all, wondering if anyone knows of a user friendly and esy to use program for generating topology diagrams for proteins. Any help would be very useful to me. thanks, Neeraj -- Neeraj Kapoor TPCB Graduate Fellow Sakmar Lab/ Molecular Biology & Biochemistry The Rockefeller Univer

[ccp4bb] Helical wheel drawing

Hi all, I am looking for a software that can help me draw a helical wheel diagram with appropriate coloring of the residues for a few peptides. Does anyone knows a cool program for the same. thanks Neeraj -- Neeraj Kapoor TPCB Graduate Fellow Sakmar Lab/ Molecular Biology

[ccp4bb] PEG oxidation

do work i cant seem to reproduce them and its very frustrating in a way. Thanks and i look forward to some helpful suggestions Neeraj -- Neeraj Kapoor TPCB Graduate Fellow Sakmar Lab/ Molecular Biology & Biochemistry The Rockefeller University 1230 York Avenue, RRB 510 New York, NY 10021

[ccp4bb] speeding crystallisation

would be welcome as i am crawling towards getting enough crystals to make a synchroton visit:):) thanks Neeraj -- Neeraj Kapoor TPCB Graduate Fellow Sakmar Lab/ Molecular Biology & Biochemistry The Rockefeller University 1230 York Avenue, RRB 510 New York, NY 10021 lab.1.212.327.8284:fax.