[ccp4bb] Large scale mammalian expression system recommendation?

2017-10-21 Thread Nathan Pollock
Hello everyone, I wonder if there is a consensus for what is currently the best system to express transmembrane proteins in mammalian cells? I think that baculovirus transduction ("Bacmam" anf the likes) has been used historically more (?) but would like to know if the modern adenovirus systems o

Re: [ccp4bb] XDSCONV not using a keyword?

2015-04-29 Thread Nathan Pollock
r all output file formats > other > than CNS and SHELX." > > so this is not a bug but known. Pointless is probably the route to take, as > Graeme suggested. > > Regards, > Tim > > On Wed, Apr 29, 2015 at 12:47:05AM -0500, Nathan Pollock wrote: > > I am trying to

[ccp4bb] XDSCONV not using a keyword?

2015-04-28 Thread Nathan Pollock
I am trying to convert XDS file into something that CCP4 would recognize while keeping reflections unmerged. Hard to believe but it's not working: the keyword MERGE=FALSE is not recognized and the default TRUE is being used no matter what I do: * XDSCONV * (VERSION March 1, 2015 BUILT=20

[ccp4bb] Jelly body refinement?

2012-08-23 Thread Nathan Pollock
Dear experts, Could someone explain what it is exactly that jelly body refinement does? I think that I understand it intuitively but want to make sure. In the same vein, what does jelly body refinement sigma parameter control? I.e., in comparison to the default sigma = 0.02, does sigma = 0.1 make

[ccp4bb] Mr. Bump and adding FASTA to CCP4 6.3 installation?

2012-08-15 Thread Nathan Pollock
Hello, Mr. Bump "requires Fasta to be installed". Could someone tell me what needs to be done to install Fasta so that it is seen and used by CCP4 6.3 programs? Installers for previous versions, 6.1 and 6.2, also installed Fasta, for 6.3 we are supposed to download and install manually. OK, I dow