Dear all,
I am trying to fit Cd in a protein structure using Phenix. However, the
refinement returns me a result with DC (2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE)
in the validation report and gives me the missing atoms accordingly. When I
open the same structure in Coot, it shows me the ion Cd. Also,
Dear all,
Thanks for all the replies. I adjusted my volumes and stopped reactions
with 8 M urea and 25 mM EDTA. The gels now run fine :)
-Mohammad
On 05-Oct-2017 7:29 PM, "Phoebe A. Rice" wrote:
> Even though the protein should be denatured by all that urea, we find such
> gels sometimes look
Dear all,
I am working with an exonuclease and I run the digested DNA on a
8Murea-20%acrylamide gel in TBE buffer. I use the Mini-Protean BioRad
system and cast gels of about 8.6x6.5 cm dimensions with 1.5 mm thickness.
I use a 15 well comb. I run my gels at 70 V for as long as 4 hours till my
und
aff/staffpage.php?StaffID=TE
>
> Invention, my dear friends, is 93% perspiration, 6% electricity, 4%
> evaporation, and 2% butterscotch ripple. ~Willy Wonka
>
>
>
> *From: *CCP4 bulletin board on behalf of Mohammad
> Khan
> *Reply-To: *Mohammad Khan
> *Date: *Friday, 2
e maximum and minimum value ? Have you
> try to change the probe concentration?
>
> Best,
>
> Didier
>
> Le 21 juil. 2017 15:34, "Mohammad Khan" a écrit :
>
> Dear all,
>
> I am trying to measure the difference in polarization upon the binding of
> t
with Cy3, but sequence that does not bind
> to test for nonspecific, concentration dependent effects.
> I hope this helps, if you have any questions, feel free to email me!
> Best,
> Julius
>
>
> > On 21 Jul 2017, at 15:32, Mohammad Khan wrote:
> >
> > Dear all,
&
Dear all,
I am trying to measure the difference in polarization upon the binding of
the DNA to my protein. I take 1-5 nM of Cy3-labelled DNA and add varying
dilutions of my protein to it (100 microM to 1 nM). I do get a decrease in
difference of polarization with decrease in protein concentration.
On Wed, Jun 7, 2017 at 3:36 PM, Mohammad Khan
wrote:
> Dear all,
>
> I am working with an exonuclease by refolding it from inclusion bodies
> (IBs). I tried various constructs and hosts, but couldn't get it in soluble
> form.
>
> I lyse my cells using a cell disruptor
Dear all,
I am working with an exonuclease by refolding it from inclusion bodies
(IBs). I tried various constructs and hosts, but couldn't get it in soluble
form.
I lyse my cells using a cell disruptor and after solubilizing IBs with
urea, I refold the protein by rapid dilution and get an aggrega
Dear all,
I am working on a His-tag recombinant protein with two domains, which I
purify using affinity chromatography. When I set up crystallization of the
same, it gave me crystals in two different conditions- one was the complete
protein. The other just had the Domain2.
Even on SDS-PAGE, I see
structura de Macromoleculas
> Centro Nacional de Biotecnologia - CSIC
> c/Darwin 3
> E-28049 Madrid, Spain
> tel. (+34) 91 585 4616
> http://www.cnb.csic.es/~mjvanraaij
>
>
>
>
>
>
>
>
> On 20 May 2015, at 11:50, Mohammad Khan wrote:
>
> > Dear all,
&
Dear all,
Is there any good online server for the generation of topology cartoons of
proteins, where one can have a clear layout of the various secondary
structures?
I have tried Pro-Origami, but however I am not veru happy with the output.
I have used the default options. Maybe if someone can he
Dear all,
I have added hydrogens on my molecule using the Refmac option in Coot.
I now want to remove them. I tried various syntax but they didn't
work. Is there a simple way to remove them from the pdb?
Moreover, if I do my refinemnets with hydrogens in it, will it affect
my results, other than
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