[ccp4bb] Post-translational modification induced strcture

2010-03-04 Thread Miles Pufall
cture. Thanks! Miles Miles Pufall Postdoctoral Fellow, UC San Francisco 600 16th Street, Genentech Hall S-574 San Francisco, California 94158-2517

Re: [ccp4bb] DNA binding protein

2009-08-10 Thread Miles Pufall
lem with subsequent crystallization trials in presence of the bona-fide target DNA sequence. 4-Precipitation of the DNA with streptomycine sulfate. You could also loss some protein. 1 and 2 are in my opinion the less invasive soutions. Hope this helps. Best regards Pascal Egea Mile

Re: [ccp4bb] expression vector

2008-03-27 Thread Miles Pufall
roups and asymmetric units. -- Roger S. Rowlett Professor Colgate University Presidential Scholar Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: [EMAIL

Re: [ccp4bb] Check the conformation of one important amino acid

2008-02-18 Thread Miles Pufall
ROTECTED] > -- Jürgen Bosch University of Washington Dept. of Biochemistry, K-426 1705 NE Pacific Street Seattle, WA 98195 Box 357742 Phone: +1-206-616-4510 FAX: +1-206-685-7002 Web: http://faculty.washington.edu/jbosch Miles Pufall Postdoctoral Scholar Yamamoto Lab UC San Francisco C