Re: [ccp4bb] difficult P1 crystal

2010-09-30 Thread Mario Milani
ount of mols per unit > cells and surely some good NCS, you should be able to phase pretty easily. > Well, of course SeMet would be the other option... > ciao, > s > > On Sep 30, 2010, at 1:54 PM, Mario Milani wrote: > >> Dear all, >> i have a 30 kDa protein that crysta

[ccp4bb] difficult P1 crystal

2010-09-30 Thread Mario Milani
to try MR but all trials so far did not solve the structure (using balbes, molrep, phaser and epmr). Any suggestion is welcome. Thank you, Mario Milani

Re: [ccp4bb] hydrodynamic radius from atomic coordinates

2008-04-22 Thread Mario Milani
> Dear colleagues, > > Does anyone know of a program to calculate hydrodynamic radius of a > protein > from atomic coordinates? > > Thanks. > > Mike Colaneri > Hi, you can use hydropro http://leonardo.fcu.um.es/macromol/programs/hydropro/hydropro.htm. mario

[ccp4bb] refining low resolution structures

2008-04-17 Thread Mario Milani
experimental data are scarce. mario Mario Milani, PhD University of Milan Dep. of Biomol. Science and Biotech. Via Celoria 26, 20133 Milan, Italy T. +39 0250314892-4 F. +39 0250314895 http://digilander.libero.it/mario.milani/ http://users.unimi.it/biolstru/Home.html

[ccp4bb] refining low resolution structures

2008-04-17 Thread Mario Milani
Hi, it could be really useful in refining low resolution structure (with poor electron density map) to have a refinement program that can restrain phi and psi angles to follow Ramachandran plot. Does anyone know if such program exist? Thank you, mario Mario Milani, PhD University of