Dear Yuri,
I am a big fan of paratone oil (or similar oils) for freezing the "holy"
crystal. The only drawback is that after you have frozen it in the oil its
a little bit hard to recover the crystal if you want to seed from it, as
its somewhat sticky...
Best of luck!
Marc
Thanks a lot to everyone for their insightful comments I certainly had
an interesting day trying to digest all that was said! Although my initial
reaction to the request was to turn it down since I had never heard of
such a request before, I decided in the end to accede to the request,
since not
to do this before I feel just a tad uncomfortable
about handing this over…
Your opinions would be greatly appreciated.
Best wishes
Marc
Dr. Marc Kvansakul
Laboratory Head, NHMRC CDA Fellow
Dept. of Biochemistry| La Trobe University | Bundoora
Rm 218, Phys Sci Bld 4, Kingsbury Drive, Melbourne
Hi Andre,
Majorie Harding wrote a few nice papers on metal ion binding sites and their
associated coordination geometry, examples are:
Harding MM (2006) Acta D vol. 62 pp. 678-82 or Harding MM (2004) Acta D vol. 60
pp. 849-59
Best
Marc
Dr. Marc Kvansakul
Laboratory Head, NHMRC CDA Fellow
SUMMARY DESCRIPTION: Structural biology of virus-host interactions
The Kvansakul Laboratory is seeking a postdoctoral fellow to investigate
the structural basis of inhibition of programmed cell death during viral
infection (e.g. Kvansakul et al 2007 Mol. Cell 25: 933 942, Kvansakul et
al Cell
Dear Atul,
Whilst finding Se-Met conditions you could always just phase with mercury if
you are lucky enough to have an accessible free Cys residue or try NaI or I3C
soaks. Always worth trying if you have readily reproducible crystals…
Best of luck
Marc
From: atul kumar mailto:atulsingh21...
Dear BBlers,
Would the kind donor of the prescission protease plasmid be willing to
share another round? I would love to make this stuff in the lab...
Best wishes
Marc
On 26/05/11 12:04 PM, "Lieh Yoon Low" wrote:
>Dear All,
>Thanks to all who replied me so quickly. In case you do not know
